Product Name	KISS1
Chinese Name	Tumour转移抑制基因抗体
Alias	KISS1_HUMAN; Metastasis-suppressor KiSS-1; Kisspeptin-1; Metastin; Kisspeptin-54; Kisspeptin-14; Kisspeptin-13; Kisspeptin-10; PP5098; Kisspeptin;
literatures	Specific References  (4)     |     SL0749R has been referenced in 4 publications. [IF=4.736] Lu Xiaosheng. et al. Deficiency of C1QL1 reduced murine ovarian follicle reserve through intraovarian and endocrine control. ENDOCRINOLOGY. 2022 Apr;:  IHC ;  Mouse.   PubMed:10.1210/endocr/bqac048 [IF=3.69] Meng-fan Peng. et al. Effects of total flavonoids from Eucommia ulmoides Oliv. leaves on polycystic ovary syndrome with insulin resistance model rats induced by letrozole combined with a high-fat diet. J Ethnopharmacol. 2021 Jun;273:113947  IHC ;  Rat.   PubMed:33617969 [IF=1.514] Liu T et al. Di-(2-ethylhexyl) phthalate induces precocious puberty in adolescent female rats.Iran J Basic Med Sci. 2018 Aug;21(8):848-855.  IHC-P ;  Rat.   PubMed:30186573 [IF=0] Yun, Sung Gyu, et al. "The Effects of Different Wavelengths of Light-Emitting Diodes on the Expression of Reproduction-Related Genes in Goldfish Carassius auratus." Fisheries and Aquatic Sciences (2015).  WB ;   PubMed:DOI:10.5657/FAS.2015.00211
Research Area	Tumour  Cell biology  Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Cow, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	16kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Kiss-1: 81-145/145
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may inhibit chemotaxis and invasion and thereby attenuate metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A protein product of this gene, kisspeptin, stimulates gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulates the pubertal activation of GnRH nuerons. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues. [provided by RefSeq, Mar 2012] Function: Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Subcellular Location: Secreted. Tissue Specificity: Very high expression in placenta, with the next highest level in testis and moderate levels in pancreas, liver, small intestine and brain at much lower levels. Expression levels increased in both early placentas and molar pregnancies and are reduced in choriocarcinoma cells. Expressed at higher levels in first trimester trophoblasts than at term of gestation, but only expressed in the villous trophoblast. Post-translational modifications: Processed by MMP2 and MMP9. Similarity: Belongs to the KISS1 family. SWISS: Q15726 Gene ID: 3814 Database links: Entrez Gene: 3814 Human Entrez Gene: 289023 Rat Omim: 603286 Human SwissProt: Q15726 Human SwissProt: Q7TSB7 Rat Unigene: 95008 Human Unigene: 66008 Rat Kiss-1主要用于Tumour及Tumour转移方面的研究。近年来Kiss-1与MMP-9、MMP-2 是Tumour转移、浸润研究的热门课题。
Product Picture	Sample: Lane 1: Human HeLa cell lysates Lane 2: Human SW480 cell lysates Lane 3: Human DU145 cell lysates Primary: Anti- KISS1 (SL0749R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 16 kDa Observed band size: 13 kDa Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Kisspeptin) Polyclonal Antibody, Unconjugated (SL0749R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Kisspeptin) Polyclonal Antibody, Unconjugated (SL0749R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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