Product Name	Galectin 3
Chinese Name	半乳糖凝集素3抗体
Alias	Galectin-3; 35 kDa lectin; Carbohydrate binding protein 35; CBP 35; CBP35; GAL 3; GAL3; GAL-3; Galactose specific lectin 3; Galactose-specific lectin 3; Galactoside binding protein; Galactoside-binding protein; GALBP; Galectin3 internal gene,included; GALIG; GBP; IgE binding protein; IgE-binding protein; L 31; L 34; L-34 galactoside-binding lectin; L31; Laminin binding protein; Laminin-binding protein; Lectin galactose binding soluble 3; Lectin galactoside binding soluble 3; Lectin L 29; Lectin L-29; Lectin, galactose binding, soluble 3; LGALS 3; LGALS2; LGALS3; LGALS3 protein; MAC 2 antigen; Mac-2; MAC2; Macrophage galactose-specific lectin; MGC105387; LEG3_HUMAN.
literatures	Specific References  (5)     |     SL0721R has been referenced in 5 publications. [IF=5.36] White, Michael JV, David Roife, and Richard H. Gomer. "Galectin-3 Binding Protein Secreted by Breast Cancer Cells Inhibits Monocyte-Derived Fibrocyte Differentiation." The Journal of Immunology (2015): 1500365.  other ;  Human.   PubMed:26136428 [IF=3.218] Liu H et al. Dual-signal sandwich-type electrochemical immunoassay of galectin-3 using methylene blue and gold nanoparticles biolabels. Journal of Electroanalytical Chemistry, 113952 (2020).  Other ;  Human.   PubMed:doi:10.1016/j.jelechem.2020.113952 [IF=2.824] Xu Mei. et al. Trichinella spiralis co-infection exacerbates Plasmodium berghei malaria-induced hepatopathy. Parasite Vector. 2020 Dec;13(1):1-16  IHC ;  Mouse.   PubMed:32883347 [IF=2.663] Zeng Y et al. Bitter melon (Momordica charantia) attenuates atherosclerosis in apo-E knock-out mice possibly through reducing triglyceride and anti-inflammation.(2018) Lipids in Health and Disease, Nov 6;17(1):251.  IHC ;  Mouse.   PubMed:30400958 [IF=1.08] Lee, Ju Yeon, et al. "Molecular cloning and expression analysis of pig CD7." Veterinary Research Communications (2014): 1-7.  Pig.   PubMed:24792331
Research Area	Tumour  immunology  Apoptosis  Cell adhesion molecule  Cell Surface Molecule
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	29kDa
Cellular localization	The nucleus cytoplasmic The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Galectin 3: 151-250/250
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the galectin family of carbohydrate binding proteins. Members of this protein family have an affinity for beta-galactosides. The encoded protein is characterized by an N-terminal proline-rich tandem repeat domain and a single C-terminal carbohydrate recognition domain. This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. This protein plays a role in numerous cellular functions including apoptosis, innate immunity, cell adhesion and T-cell regulation. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]. Function: Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Subunit: Probably forms homo- or heterodimers. Interacts with DMBT1 (By similarity). Forms a complex with the ITGA3, ITGB1 and CSPG4. Interacts with LGALS3BP, LYPD3, CYHR1 and UACA. Subcellular Location: Cytoplasm. Nucleus. Secreted. Note=Secreted by a non-classical secretory pathway and associates with the cell surface. Tissue Specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Similarity: Contains 1 galectin domain. SWISS: P17931 Gene ID: 3958 Database links: Entrez Gene: 3958 Human Entrez Gene: 16854 Mouse Entrez Gene: 83781 Rat Omim: 153619 Human SwissProt: P17931 Human SwissProt: P16110 Mouse SwissProt: P08699 Rat Unigene: 531081 Human Unigene: 248615 Mouse Unigene: 764 Rat Galectin-3是Galectin（半乳凝素）家族的一员，能识别glycoprotein和糖脂的特异性低聚糖结构，参与多种生理和病理过程，包括细胞生长和凋亡、细胞黏附、新生血管形成和Tumour浸润与转移等。Galectin-3在Tumour的发生、发展及转移中起重要作用。 Galectin-3在某些Tumour组织中高表达，其在不同类型Tumour，如结直肠癌、胃癌、胰腺癌、甲状腺癌及肺癌等患者中的高表达水平，与低生存、Tumour复发、预后差及对化疗药物敏感性降低相关对于某些Tumour，Galectin-3有可能成为一个有价值的诊断标志和治疗靶点。
Product Picture	Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Galectin 3 Polyclonal Antibody, Unconjugated(SL0721R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (SL0721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (SL0721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (SL0721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (SL0721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (SL0721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (SL0721R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Cervical Cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Galectin 3) Polyclonal Antibody, Unconjugated (SL0721R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Galectin 3 Polyclonal Antibody, Unconjugated(SL0721R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control:A431. Primary Antibody (green line): Rabbit Anti-Galectin 3 antibody (SL0721R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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