Product Name	CD8
Chinese Name	CD8抗体
Alias	CD8 antigen, alpha polypeptide (p32); CD8a; CD8a antigen; CD8a molecule; CD8A_MOUSE; CD8A_HUMAN; Leu2; Leu2 T lymphocyte antigen; MAL; OKT8 T cell antigen; p32; T cell antigen Leu2; T cell co receptor; T-cell surface glycoprotein CD8 alpha chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T cell antigen.
literatures	Specific References  (71)     |     SL0648R has been referenced in 71 publications. [IF=29.497] Di Zhao. et al. Chromatin Regulator CHD1 Remodels the Immunosuppressive Tumor Microenvironment in PTEN-Deficient Prostate Cancer. Cancer Discov. 2020 Sep;10(9):1374-1387  IF,IHC ;  Human.   PubMed:32385075 [IF=16.907] Senbiao Chenaet al. Carrier-free nanoassembly of doxorubicin prodrug and siRNA for combinationally inducing immunogenic cell death and reversing immunosuppression. Nano Today 35 (2020) 100924  IHC ;  mouse.   PubMed:10.1016/j.nantod.2020.100924 [IF=15.304] Jinbo Li. et al. Autophagy inhibition recovers deficient ICD-based cancer immunotherapy. BIOMATERIALS. 2022 Aug;287:121651  IF ;  Mouse.   PubMed:35777331 [IF=13.801] Kang Kyung-Don. et al. Safety and efficacy of intraventricular immunovirotherapy with oncolytic HSV-1 for CNS cancers. CLIN CANCER RES. 2022 Oct;:  IHC ;  Mouse.   PubMed:36239623 [IF=13.281] Yao Li. et al. Antigen Capture and Immune Modulation by Bacterial Outer Membrane Vesicles as In Situ Vaccine for Cancer Immunotherapy Post-Photothermal Therapy. 2022 Feb 12  IF ;  Mouse.   PubMed:35152555 [IF=12.779] Lenz, Max. et al. Pharmacologic modulation of intracellular Na+ concentration with ranolazine impacts inflammatory response in humans and mice. P NATL ACAD SCI USA. 2022 Jul;119(29):e2207020119  IF ;  Mouse.   PubMed:35858345 [IF=11.467] Shuang Zhou. et al. Tumor microenvironment adrenergic nerves blockade liposomes for cancer therapy. J CONTROL RELEASE. 2022 Nov;351:656  IF ;  Mouse.   PubMed:36183971 [IF=11.467] Xudong Wen. et al. A macrophage membrane-coated mesoporous silica nanoplatform inhibiting adenosine A2AR via in situ oxygen supply for immunotherapy. J CONTROL RELEASE. 2023 Jan;353:535  IF ;  Mouse.   PubMed:36481693 [IF=10.479] Christophe Glorieux. et al. Cisplatin and gemcitabine exert opposite effects on immunotherapy with PD-1 antibody in K-ras-driven cancer. J Adv Res. 2021 Dec;:  IHC ;  Human,Mouse.   PubMed:10.1016/j.jare.2021.12.005 [IF=10.435] Wan, Guoyun. et al. Gene augmented nuclear-targeting sonodynamic therapy via Nrf2 pathway-based redox balance adjustment boosts peptide-based anti-PD-L1 therapy on colorectal cancer. J Nanobiotechnol. 2021 Dec;19(1):1-26  IF ;  Mouse.   PubMed:34715867 [IF=9.986] Christophe Glorieux. et al. Regulation of PD-L1 expression in K-ras-driven cancers through ROS-mediated FGFR1 signaling. Redox Biol. 2021 Jan;38:101780  IHC ;  Mouse.   PubMed:33171331 [IF=9.273] Qing Yao. et al. Water-responsive gel extends drug retention and facilitates skin penetration for curcumin topical delivery against psoriasis. ASIAN J PHARM SCI. 2023 Feb;:100782  IF ;  Mouse.   PubMed:36845839 [IF=8.806] Li TF et al. Dendritic cell-mediated delivery of doxorubicin-polyglycerol-nanodiamond composites elicits enhanced anti-cancer immune response in glioblastoma.Biomaterials. 2018 Oct;181:35-52.  IHC-P ;  Human.   PubMed:30071380 [IF=8.39] Wang, Raymond M., et al. "Humanized mouse model for assessing the human immune response to xenogeneic and allogeneic decellularized biomaterials." Biomaterials (2017).  IHC ;  Mouse.   PubMed:28334641 [IF=8.128] Xinwei Kuang. et al. Ag nanoparticles enhance immune checkpoint blockade efficacy by promoting of immune surveillance in melanoma. J Colloid Interf Sci. 2022 Jun;616:189  IF ;  Mouse.   PubMed:10.1016/j.jcis.2022.02.050 [IF=7.786] Zhu,et al.Co-delivery of tumor antigen and dual toll-like receptor ligands into dendritic cell by silicon microparticle enables efficient immunotherapy against melanoma.(2018) Journal of Controlled Release. 272:72-82.  IHC-P ;  Mouse.   PubMed:29325699 [IF=7.727] Jinbo Li. et al. Low dose shikonin and anthracyclines coloaded liposomes induce robust immunogenetic cell death for synergistic chemo-immunotherapy. J Control Release. 2021 Jul;335:306  IHC ;  Mouse.   PubMed:34081995 [IF=7.367] Yitian Du. et al. Engineered Microglia Potentiate the Action of Drugs against Glioma Through Extracellular Vesicles and Tunneling Nanotubes. 2021 Feb 28  IF,IHC ;  Mouse.   PubMed:33644993 [IF=7.182] Chengxiao Wang. et al. Dissolvable microneedles based on Panax notoginseng polysaccharide for transdermal drug delivery and skin dendritic cell activation. Carbohyd Polym. 2021 May;:118211  IF ;  Mouse.   PubMed:34127215 [IF=7.068] Wenli Fang. et al. Progranulin induces immune escape in breast cancer via up-regulating PD-L1 expression on tumor-associated macrophages (TAMs) and promoting CD8 + T cell exclusion. J Exp Clin Canc Res. 2021 Dec;40(1):1-11  IF ;  Mouse.   PubMed:33390170 [IF=6.633] Manuel Salzmann. et al. Staphylococcus aureus extracellular adherence protein (Eap) reduces immune cell phenotype in developing but not in established atherosclerotic lesions. BBA-MOL BASIS DIS. 2022 Dec;:166616  IF ;  Mouse.   PubMed:36513287 [IF=6.493] Ashraf et al. Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation. (2016) Am.J.Transplant. 16:808-20  IHC-P ;  Mouse.   PubMed:26595644 [IF=6.28] Kroner, Jochen, et al. "Mast cells are critical regulators of bone fracture‐induced inflammation and osteoclast formation and activity." Journal of Bone and Mineral Research (2017).  IHC-P ;  Mouse.   PubMed:28777474 [IF=6.081] Long-Yi Chan. et al. Cre/LoxP Genetic Recombination Sustains Cartilage Anabolic Factor Expression in Hyaluronan Encapsulated MSCs Alleviates Intervertebral Disc Degeneration. Biomedicines. 2022 Mar;10(3):555  IHC ;  Rat.   PubMed:10.3390/biomedicines10030555 [IF=6.055] Melanie R Kuhn. et al. Myeloid cell-derived catecholamines influence bone turnover and regeneration in mice. FRONT ENDOCRINOL. 2022 Sep 15;13:997745  IHC ;  Mouse.   PubMed:36187089 [IF=5.955] Weiqi Meng. et al. ZhenQi FuZheng formula inhibits the growth of colorectal tumors by modulating intestinal microflora-mediated immune function. AGING-US. 2022 Jun 15; 14(11): 4769–4785  IHC ;  Mouse.   PubMed:35680568 [IF=5.955] Weiqi Meng. et al. ZhenQi FuZheng formula inhibits the growth of colorectal tumors by modulating intestinal microflora-mediated immune function. AGING-US. 2022 Jun 15; 14(11): 4769–4785  WB ;  Mouse.   PubMed:35680568 [IF=5.738] Shui-Qing Bi. et al. Combined treatment with epigenetic agents enhances anti-tumor activity of MAGE-D4 peptide-specific T cells by upregulating the MAGE-D4 expression in glioma.. FRONT ONCOL. 2022 Aug;12:873639-873639  IHC ;  Mouse.   PubMed:35992806 [IF=5.68] Ashraf, M. I., et al. "Exogenous Lipocalin 2 Ameliorates Acute Rejection in a Mouse Model of Renal Transplantation." American Journal of Transplantation (2015).  IHC-P ;  Mouse.   PubMed:26595644 [IF=5.595] Rapp et al. Induced global deletion of glucocorticoid receptor impairs fracture healing. (2018) FASEB.J. 32:2235-2245  IHC ;  Mouse.   PubMed:29217668
Research Area	Cell biology  immunology  Stem cells  Cell Surface Molecule  Cell type markers  lymphocyte  t-lymphocyte
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	27kDa
Detection molecular weight	32 kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse CD8: 51-150/247 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]. Function: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains. Subunit: In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P01731 Gene ID: 12525 Database links: Entrez Gene: 925 Human Entrez Gene: 12525 Mouse SwissProt: P01732 Human SwissProt: P01731 Mouse
Product Picture	Sample: Lane 1: mCD8a overexpression 293T Lysate at 8 ug Primary: Anti-CD8a (SL0648R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35-42 kD Observed band size: 41 kD Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti- CD8 (SL0648R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 36 kD Sample:Lymph node(Mouse) Lysate at 30 ug Primary: Anti-CD8(SL0648R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 32 kD Image kindly submitted by Amy Beck as part of the free sample program. Formalin-fixed and paraffin embedded mouse spleen labeled with Rabbit Anti-CD8 Polyclonal Antibody, Unconjugated (SL0648R) at 1:250.

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