Product Name	Dopamine beta Hydroxylase
Chinese Name	多巴胺β羟化酶抗体
Alias	DBH; DBM; Dopamine beta hydroxylase; Dopamine-Beta-Hydroxylase; Dopamine beta monooxygenase; DOPO_RAT; DOPO_HUMAN.
Research Area	Tumour  Neurobiology  Synthesis and Degradation
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	70kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human DBH: 471-550/621
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Dopamine-beta-Hydroxylase (DBH), Dopamine can be transformed into Noradrenalin. Conversion of dopamine to noradrenaline. [SUBUNIT] Homotetramer composed of two non-covalently bound disulfide-linked dimers. [SUBCELLULAR LOCATION] Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen. Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen. Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (Potential). Belongs to the copper type II ascorbate-dependent monooxygenase family. Function: Conversion of dopamine to noradrenaline. Subunit: Homotetramer composed of two non-covalently bound disulfide-linked dimers (By similarity). Subcellular Location: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle, secretory vesicle lumen (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen (By similarity). Cytoplasmic vesicle, secretory vesicle membrane; Single-pass type II membrane protein (By similarity). Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane; Single-pass type II membrane protein (By similarity). Tissue Specificity: Chromaffin granules of the adrenal medulla and synaptic vesicles of the sympathetic nervous system. Similarity: Belongs to the copper type II ascorbate-dependent monooxygenase family. Contains 1 DOMON domain. SWISS: P09172 Gene ID: 1621 Database links: Entrez Gene: 280758 Cow Entrez Gene: 1621 Human Entrez Gene: 13166 Mouse Entrez Gene: 25699 Rat Omim: 223360 Human Omim: 609312 Human SwissProt: P15101 Cow SwissProt: P09172 Human SwissProt: Q64237 Mouse SwissProt: Q05754 Rat Unigene: 223858 Human Unigene: 591890 Human Unigene: 167781 Mouse Unigene: 87166 Rat Dopamine-β-Hydroxylase（DBH）—多巴胺β羟化酶。可以将多巴胺转化成去甲肾上腺素。是肾上腺素能神经元的标志。
Product Picture	Sample: Jurkat (Human) Cell Lysate at 40 ug Primary: Anti-Dopamine beta Hydroxylase (SL0596R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 70 kD Observed band size: 70 kD Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (DOPO) Polyclonal Antibody, Unconjugated (SL0596R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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