Product Name	CD24
Chinese Name	CD24抗体
Alias	Signal transducer CD24; CD 24; CD24 antigen (small cell lung carcinoma cluster 4 antigen); CD24 antigen; CD24 molecule; CD24A; GPI linked surface mucin; Heat stable antigen; HSA; Nectadrin; Small cell lung carcinoma cluster 4 antigen; Ly-52; CD24_HUMAN.
literatures	Specific References  (4)     |     SL0528R has been referenced in 4 publications. [IF=4.103] Qi Sun. et al. Denosumab alleviates intervertebral disc degeneration adjacent to lumbar fusion by inhibiting endplate osteochondral remodeling and vertebral osteoporosis in ovariectomized rats. Arthritis Res Ther. 2021 Dec;23(1):1-16  IHC ;  Rat.   PubMed:34049577 [IF=3.23] Khan, Mohammed I., et al. "Comparative Gene Expression Profiling of Primary and Metastatic Renal Cell Carcinoma Stem Cell-Like Cancer Cells." PLOS ONE 11.11 (2016): e0165718.  IF(ICC) ;  Human.   PubMed:27812180 [IF=3.17] Szafarowski T et al. Assessment of cancer stem cell marker expression in primary head and neck squamous cell carcinoma shows prognostic value for aldehyde dehydrogenase (ALDH1A1). Eur J Pharmacol. 2020 Jan 15;867:172837.  IHF-P ;  Human.   PubMed:31811857 [IF=1.56] Wang, Xuming, et al. "Characterization of sphere‑forming cells with stem‑like properties from the gastric cancer cell lines MKN45 and SGC7901." Molecular Medicine Reports 10.6 (2014): 2937-2941.  FCM ;  Human.   PubMed:25270642
Research Area	Tumour  Cell biology  immunology  Neurobiology  Stem cells  Cell adhesion molecule  Cell Surface Molecule  glycoprotein  t-lymphocyte  b-lymphocyte  marrow cells
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 Flow-Cyt=1μg /test  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	8kDa
Detection molecular weight	35-45kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD24 Propeptide: 60-80/80
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a sialoglycoprotein that is expressed on mature granulocytes and B cells and modulates growth and differentiation signals to these cells. The precursor protein is cleaved to a short 32 amino acid mature peptide which is anchored via a glycosyl phosphatidylinositol (GPI) link to the cell surface. This gene was missing from previous genome assemblies, but is properly located on chromosome 6. Non-transcribed pseudogenes have been designated on chromosomes 1, 15, 20, and Y. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] Function: Modulates B-cell activation responses. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. Subcellular Location: Cell membrane. Tissue Specificity: B-cells. Expressed in a number of B-cell lines including P32/SH and Namalwa. Expressed in erythroleukemia cell and small cell lung carcinoma cell lines. Also expressed on the surface of T-cells. Post-translational modifications: Extensively O-glycosylated. DISEASE: Genetic variations in CD24 are associated with susceptibility to multiple sclerosis (MS). A multifactorial, inflammatory, demyelinating disease of the central nervous system. Sclerotic lesions are characterized by perivascular infiltration of monocytes and lymphocytes and appear as indurated areas in pathologic specimens (sclerosis in plaques). The pathological mechanism is regarded as an autoimmune attack of the myelin sheat, mediated by both cellular and humoral immunity. Clinical manifestations include visual loss, extra-ocular movement disorders, paresthesias, loss of sensation, weakness, dysarthria, spasticity, ataxia and bladder dysfunction. Genetic and environmental factors influence susceptibility to the disease. Note=Polymorphisms in CD24 may act as a genetic modifier for susceptibility and progression of MS in some populations, perhaps by affecting the efficiency of CD24 expression on the cell surface. Similarity: Belongs to the CD24 family. SWISS: P25063 Gene ID: 100133941 Database links: Entrez Gene: 100133941 Human Entrez Gene: 12484 Mouse Entrez Gene: 25145 Rat Omim: 600074 Human SwissProt: P25063 Human SwissProt: P24807 Mouse SwissProt: Q07490 Rat Unigene: 644105 Human Unigene: 721443 Human Unigene: 29742 Mouse Unigene: 6007 Rat 神经Stem cellsMaker TumourMaker CD24是一种低分子质量高度糖基化的黏附分子, 主要表达在造血系统. 最近研究表明, 在造血系统Tumour和一些器官的实体瘤表面可见CD24高表达。 近几年很多学者认为：CD24在前列腺癌、非小细胞肺癌、鼻咽癌、原发性肝细胞癌、胆管细胞癌、麦克尔细胞癌、神经源性Tumour、胰腺癌、胃癌、结肠癌、肾细胞癌、B细胞淋巴瘤、乳腺癌等Tumour组织中表达增强, 且和Tumour侵袭转移、复发和预后相关。
Product Picture	Sample: Colon carcinoma (Human) lysate at 30ug; Heart(Rat) lysates, 30ug; Primary: Anti-CD24 (SL0528R) at 1:200 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000; Predicted band size : 8kD Observed band size : 35kD Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CD24 Polyclonal Antibody, Unconjugated(SL0528R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: A549 (blue). Primary Antibody:Rabbit Anti-CD24 antibody(SL0528R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (SL0528R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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