Product Name	ATF3
Chinese Name	活化转录因子3抗体
Alias	Activating transcription factor 3; ATF3deltaZip2; ATF3deltaZip2c; ATF3deltaZip3; cAMP dependent transcription factor ATF3; Cyclic AMP dependent transcription factor ATF3; Cyclic AMP-dependent transcription factor ATF-3; TF3_HUMAN; cAMP-dependent transcription factor ATF-3; cyclic AMP-dependent transcription factor ATF-3 isoform 1.
literatures	Specific References  (10)     |     SL0519R has been referenced in 10 publications. [IF=12.38] Smith, Carolyne K., et al. "Lupus high-density lipoprotein induces proinflammatory responses in macrophages by binding lectin-like oxidised low-density lipoprotein receptor 1 and failing to promote activating transcription factor 3 activity." Annals of the Rheumatic Diseases (2016): annrheumdis-2016.  FCM ;  Human.   PubMed:27543414 [IF=9.756] Ramona Palombo. et al. Inhibition of the PI3K/AKT/mTOR signaling promotes an M1 macrophage switch by repressing the ATF3-CXCL8 axis in Ewing sarcoma. CANCER LETT. 2023 Feb;555:216042  ChIP ;  Human.   PubMed:36565919 [IF=8.724] Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039  WB ;  Mouse.   PubMed:33511306 [IF=4.77] Aguirre, Alina, et al. "Defective autophagy impairs ATF3 activity and worsens lung injury during endotoxemia." Journal of Molecular Medicine (2014): 1-12.  IHC-P ;  Mouse.   PubMed:24535031 [IF=4.2] Yuan, Qing, et al. "Docetaxel-loaded solid lipid nanoparticles suppress breast cancer cells growth with reduced myelosuppression toxicity." International Journal of Nanomedicine 9 (2014): 4829.  WB ;  Mouse.   PubMed:25378924 [IF=3.73] Hu, Jianran, Yu Li, and Ping Li. "MARVELD1 Inhibits Nonsense-Mediated RNA Decay by Repressing Serine Phosphorylation of UPF1." PLOS ONE 8.6 (2013): e68291.  WB ;  Human.   PubMed:23826386 [IF=3.352] Rongxue Wei. et al. Study on the Effect of Different Types of Sugar on Lipid Deposition in Goose Fatty Liver. Poultry Sci. 2022 Jan;:101729  WB ;  Goose.   PubMed:35172237 [IF=2.965] Magadmi Rania M.. et al. The Neuroprotective Effect of Carvedilol on Diabetic Neuropathy: An In Vitro Study. J Diabetes Res. 2021;2021:6927025   PubMed:33532503 [IF=2.24] Feng, J. et al. "Upregulation of ATF-3 is correlated with prognosis and proliferation of laryngeal cancer by regulating Cyclin D1 expression" International Journal of Clinical and Experimental Pathology  2013;6(10):2064-2070  other ;   PubMed:24133584 [IF=1.978] Xu HY et al. Bovine bta-microRNA-1271 Promotes Preadipocyte Differentiation by Targeting Activation Transcription Factor 3. Biochemistry (Mosc). 2020 Jul;85(7):749-757.  WB ;  Cow&Human.   PubMed:33040719
Research Area	Tumour  Cell biology  immunology  Signal transduction  Apoptosis  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Chicken, Dog, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	20kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ATF3: 51-150/181
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]. Function: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Subunit: Binds DNA as a homodimer or a heterodimer. Subcellular Location: Nucleus. Similarity: Belongs to the bZIP family. ATF subfamily. Contains 1 bZIP domain. SWISS: P18847 Gene ID: 467 Database links: Entrez Gene: 467 Human Entrez Gene: 11910 Mouse Entrez Gene: 25389 Rat Omim: 603148 Human SwissProt: P18847 Human SwissProt: Q60765 Mouse SwissProt: P29596 Rat Unigene: 460 Human Unigene: 2706 Mouse Unigene: 9664 Rat transcriptional regulatory factor（Transcriptin Regulators） 活化转录因子-3(属于activation transcription factor/cAMP responsive element binding protein家族)是真核细胞转录因子，同属于ATF/CREB家族。参与由cAMP或某些病毒蛋白质所诱导基因转录的调节。 转录激活因子3(activating transcription factor 3,ATF3)属含亮氨酸拉链结构的转录因子ATF/CREB家族成员，分子量20kDa,在大部分正常细胞中呈低浓度稳态表达。在正常组织中含量很低，在应激状态是表达增加，形成二聚体。ATF3是个应激早期快反应基因，体内外可被一系列损伤性应激信号快速诱导表达。ATF3不仅在应激反应中是个关键性的调控因子，还广泛参与了机体稳态维持、创伤愈合、细胞黏附、Tumour形成、凋亡以及信号传导等生理和病理过程。ATF3与细胞生存和凋亡有关，但在不同的细胞状态下发挥不同甚至相反的作用。
Product Picture	Sample: Embryo(Mouse) lysate 30ug; Primary: Anti-ATF3 (SL0519R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000; Predicted band size : 20kD Observed band size : 19kD Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATF3 Polyclonal Antibody, Unconjugated(SL0519R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATF3 Polyclonal Antibody, Unconjugated(SL0519R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATF3 Polyclonal Antibody, Unconjugated(SL0519R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon carcinoma;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ATF3 Polyclonal Antibody, Unconjugated(SL0519R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei

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