Product Name	ADRB1
Chinese Name	肾上腺素能受体β1抗体
Alias	beta 1 Adrenergic Receptor; beta 1 Adrenergic Receptor; ADRB1R; Adrenergic beta 1 receptor; B1AR; Beta 1 adrenoceptor; Beta 1 adrenoreceptor; Beta-1 adrenergic receptor; Beta-1 adrenoceptor; Beta-1 adrenoreceptor; BETA1AR; RHR; ADR B1; ADRB 1; ADRB1; ADRB1_HUMAN; ADRB1R; Adrenergic beta 1 receptor; Adrenoceptor beta 1.
literatures	Specific References  (8)     |     SL0498R has been referenced in 8 publications. [IF=6.208] Lina S. Farhoumand. et al. Blockade of ß-Adrenergic Receptors by Nebivolol Enables Tumor Control Potential for Uveal Melanoma in 3D Tumor Spheroids and 2D Cultures. INT J MOL SCI. 2023 Jan;24(6):5894  FCM ;  Human.   PubMed:36982966 [IF=4.831] Liu Z et al. Over-expression of microRNA-145 drives alterations in β-adrenergic signaling and attenuates cardiac remodeling in heart failure post myocardial infarction. Aging (Albany NY). 2020 Jun 18;12(12):11603-11622.  WB ;  Rat.   PubMed:32554856 [IF=3.727] Yue Wang. et al. Mirabegron Ameliorated Atherosclerosis of ApoE−/− Mice in Chronic Intermittent Hypoxia but Not in Normoxia. 2021 Jun 21  IHC ;  Mouse.   PubMed:34152510 [IF=3.14] Tibenska V et al. Gradual cold acclimation induces cardioprotection without affecting beta-adrenergic receptor-mediated adenylyl cyclase signaling. J Appl Physiol (1985). 2020 Apr 1;128(4):1023-1032.  WB ;  rat.   PubMed:32213114 [IF=3.07] Deng, Guo-Hua, et al. "Exogenous norepinephrine attenuates the efficacy of sunitinib in a mouse cancer model." Journal of Experimental & Clinical Cancer Research 33:21 (2014).  IHC-P ;  Mouse.   PubMed:24555849 [IF=2.71] Bornholz, Beatrice, et al. "A standardised FACS assay based on native, receptor transfected cells for the clinical diagnosis and monitoring of β1-adrenergic receptor autoantibodies in human heart disease." Clinical Chemistry and Laboratory Medicine (CCLM).  Human.   PubMed:26408610 [IF=2.5] Jinyao Liuet al. Alcohol consumption combined with dietary low-carbohydrate/high-protein intake increased the left ventricular systolic dysfunction risk and lethal ventricular arrhythmia susceptibility in apolipoprotein E/low-density lipoprotein receptor double-knockout mice. Alcohol . 2020 Dec;89:63-74.  IHC ;  mouse.   PubMed:32702503 [IF=2.078] Shi L et al. β1 adrenoceptor antibodies induce myocardial apoptosis via inhibiting PGC-1α-related pathway. BMC Cardiovasc Disord. 2020 Jun 5;20(1):269.  WB ;  Rat.   PubMed:32503464
Research Area	Cardiovascular  immunology  Neurobiology  Signal transduction  Growth factors and hormones  Channel protein  The cell membrane受体
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	52kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ADRB1: 181-250/477 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Beta-adrenergic receptors (Beta-1 adrenoreceptor) mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Subcellular location cell membrane; Multi-pass membrane protein. Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. Belongs to the G-protein coupled receptor 1 family. Function: Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Subunit: Interacts with GOPC, MAGI3 and DLG4. Subcellular Location: Cell membrane; Multi-pass membrane protein. Note=Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression. Post-translational modifications: Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. Similarity: Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily. SWISS: P08588 Gene ID: 153 Database links: Entrez Gene: 153 Human Entrez Gene: 11554 Mouse Entrez Gene: 24925 Rat Omim: 109630 Human SwissProt: P08588 Human SwissProt: P34971 Mouse SwissProt: P18090 Rat Unigene: 99913 Human Unigene: 46797 Mouse Unigene: 87064 Rat β1肾上腺素能受体（Beta-adrenergic Receptor-1 ）在接受交感神经节后纤维支配的各种器官中存在着与肾上腺素、去甲肾上腺素起反应的受体，称为肾上腺素能受体。其化学性质尚不清楚。用对药物反应的方法，肾上腺素能受体可分为α及β两个类型。肾上腺素对α、β及D型受体均起作用，而去甲肾上腺素主要对α型起作用。 β1AR所引起的反应为支气管扩张、血管扩张等，肾上腺素受体可被β13，4－dichloroisoproterenol（D－CI）等阻断药物所抑制。
Product Picture	Sample: Heart (Mouse) Lysate at 30 ug Heart (Rat) Lysate at 30 ug Primary: Anti- ADRB1(SL0498R) at 1/200 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/3000 dilution Predicted band size: 52 kD Observed band size: 55 kD Tissue/cell: skin of rat foot; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(SL0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ADRB1 Polyclonal Antibody, Unconjugated(SL0498R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control:U937. Primary Antibody (green line): Rabbit Anti- antibody (SL3152R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells wereincubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control:U937. Primary Antibody (green line): Rabbit Anti-ADRB1 antibody (SL0498R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Tissue/cell: mouse kidney cell; Incubation: Red line: Anti-ADRB1 Polyclonal Antibody, FITC conjugated(SL0498R-FITC) 1:200, 40 minutes at 37°C. Green line: Isotype Control(SL0295P-FITC, rabbit IgG/FITC) 1:200, 40 minutes at 37°C.

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Totalgoods,subtotals:￥Checkout