Product Name	Dnmt3a
Chinese Name	DNA甲基转移酶-3α抗体
Alias	DNA (cytosine 5 ) methyltransferase 3 alpha; DNA (cytosine 5) methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaI; DNA MTase HsaIIIA; DNMT 3a; DNMT; DNMT3A2; M HsaIIIA; M.HsaIIIA; MCMT; Methyl CpG binding domain protein 3a.
literatures	Specific References  (7)     |     SL0497R has been referenced in 7 publications. [IF=8.739] Ai Wei. et al. Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis. 2021 Aug 11  WB,IHC ;  Mouse.   PubMed:34378791 [IF=8.401] Dr. xingren Chen. et al. Reversal of epigenetic PPARγ suppression by diacerein alleviates oxidative stress and osteoarthritis in mice. 2022 Feb 24  WB ;  Mouse.   PubMed:10.1089/ars.2021.0219 [IF=5.714] Li D et al. Oxygenated Polycyclic aromatic hydrocarbons (Oxy-PAHs) facilitate lung cancer metastasis by epigenetically regulating the epithelial-to-mesenchymal transition (EMT). Environ Pollut. 2019 Sep 17;255(Pt 2):113261.  WB ;  Human.   PubMed:31580991 [IF=5.2] Zhao, Qian, et al. "Prenatal Cocaine Exposure Impairs Cognitive Function of Progeny Via Insulin Growth Factor II Epigenetic Regulation." Neurobiology of Disease (2015).  WB ;  Mouse.   PubMed:26054440 [IF=3.85] Wu, Yuting, et al. "Methylation of Septin9 mediated by DNMT3a enhances hepatic stellate cells activation and liver fibrogenesis." Toxicology and Applied Pharmacology 315 (2017): 35-49.  WB ;  Mouse.   PubMed:27939986 [IF=3.337] Jiachen Wang. et al. Upregulation of miR-137 Expression Suppresses Tumor Growth and Progression via Interacting with DNMT3a Through Inhibiting the PTEN/Akt Signaling in HCC. Oncotargets Ther. 2021; 14: 165–176  WB ;  Human.   PubMed:33447058 [IF=0] Gökmen, Esra, and Kemal Ergin. "DNA Methyltransferase Expression and Proliferation Status of Metastatic Breast Cancer Cell Line After Prolonged and Repeated Rapamycin and Melatonin Application." Meandros Medical Journal.  Human.   PubMed:10.4274/meandros.2117
Research Area	Tumour  Cell biology  Signal transduction  Apoptosis  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	100kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Dnmt3a: 26-100/912
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. [provided by RefSeq, Mar 2016] Function: Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity. Subunit: Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with UBC9, PIAS1 and PIAS2. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD domain. Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Interacts with MPHOSPH8. Interacts with histone H3 that is not methylated at 'Lys-4' (H3K4). Subcellular Location: Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin. Tissue Specificity: Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. Post-translational modifications: Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription. Similarity: Belongs to the C5-methyltransferase family. Contains 1 ADD domain. Contains 1 GATA-type zinc finger. Contains 1 PHD-type zinc finger. Contains 1 PWWP domain. SWISS: Q9Y6K1 Gene ID: 1788 Database links: Entrez Gene: 1788 Human Entrez Gene: 13435 Mouse Omim: 602769 Human SwissProt: Q9Y6K1 Human SwissProt: O88508 Mouse Unigene: 515840 Human Unigene: 5001 Mouse Tumour组织存在DNA甲基化紊乱，包括与细胞增殖周期密切相关的癌基因低甲基化和抑癌基因高甲基化DNA甲基转移酶(Dnmt)参与甲基化的形成(主要是Dnmt3a和Dnmt3b)和维持（主要是Dnmt1）
Product Picture	Sample: A549(Human) Cell Lysate at 30 ug A431(Human) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Lovo(Human) Cell Lysate at 30 ug Primary: Anti-Dnmt3a (SL0497R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 120 kD Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 3: Testis (Rat) Lysate at 40 ug Lane 4: 293T (Human) Cell Lysate at 30 ug Lane 5: SH-SY5Y (Human) Cell Lysate at 30 ug Lane 6: Molt-4 (Human) Cell Lysate at 30 ug Lane 7: HepG2 (Human) Cell Lysate at 30 ug Primary: Anti-Dnmt3a (SL0497R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 120 kD Observed band size: 120 kD Sample: Embryo(Mouse) Lysate at 40 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-Dnmt3a (SL0497R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 130 kD Observed band size: 120 kD Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Dnmt3a) Polyclonal Antibody, Unconjugated (SL0497R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Dnmt3a Polyclonal Antibody, Unconjugated(SL0497R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human breast carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Dnmt3a Polyclonal Antibody, Unconjugated(SL0497R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (Black line): U87MG (Black). Primary Antibody (green line): Rabbit Anti-Dnmt3a antibody (SL0497R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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