Product Name	Ghrelin
Chinese Name	脑肠肽抗体
Alias	Appetite regulating hormone; Ghrelin 27; Ghrelin 28; Ghrelin/obestatin prepropeptide; GHRL; Growth hormone releasing peptide; Growth hormone secretagogue; M46 protein; Motilin related peptide; MTLRP; Obestatin; Obestatin preprohormone; PRO1066; UNQ524; GHRL_HUMAN.
literatures	Specific References  (8)     |     SL0467R has been referenced in 8 publications. [IF=5.29] Wölnerhanssen, Bettina K., et al. "Deregulation of transcription factors controlling intestinal epithelial cell differentiation; a predisposing factor for reduced enteroendocrine cell number in morbidly obese individuals." Scientific Reports 7 (2017).  IHC ;  Human.   PubMed:28811552 [IF=4.546] Quanwei Zhang. et al. Zearalenone Affect the Intestinal Villi Associated with the Distribution and the Expression of Ghrelin and Proliferating Cell Nuclear Antigen in Weaned Gilts. Toxins. 2021 Oct;13(10):736  WB,IHC ;  Pig.   PubMed:34679029 [IF=4.546] Quancheng Liu. et al. FumDSB Can Reduce the Toxic Effects of Fumonisin B1 by Regulating Several Brain-Gut Peptides in Both the Hypothalamus and Jejunum of Growing Pigs. Toxins. 2021 Dec;13(12):874  WB,IHC ;  Pig.   PubMed:34941712 [IF=3.23] Lemarié F, Beauchamp E, Dayot S, Duby C, Legrand P, Rioux V (2015) Dietary Caprylic Acid (C8:0) Does Not Increase Plasma Acylated Ghrelin but Decreases Plasma Unacylated Ghrelin in the Rat. PLoS ONE 10(7): e0133600.  IHC-P ;  Rat.   PubMed:26196391 [IF=2.784] Liu et al. Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B. (2018) Int.J.Mol.Med. 42:3037-3046  WB&IHC ;  Rat.   PubMed:30272367 [IF=1.77] Wang et al. Effects of Wen Dan Tang on insomnia-related anxiety and levels of the brain-gut peptide Ghrelin. (2014) Neural.Regen.Re. 9:205-12  IHC-F ;  Rat.   PubMed:25206802 [IF=1.51] Dai, Meiling, et al. "Effects of zearalenone-diet on expression of ghrelin and PCNA genes in ovaries of post-weaning piglets." Animal Reproduction Science (2016).  IHC-P ;  Pig.   PubMed:27020865 [IF=0] Armasu I et al. Ghrelin expression in breast carcinomas. Endocrine Abstracts (2019) 63 P648.  IHC ;  Human.   PubMed:doi:10.1530/endoabs.63.P648
Research Area	Cardiovascular  Neurobiology  Signal transduction  Growth factors and hormones  Diabetes  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Rat,  (predicted: Human, Mouse, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	10kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Ghrelin: 31-117/117
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes ghrelin-obestatin preproprotein, which generates ghrelin and obestatin. Ghrelin is an endogenous ligand for the growth hormone secretagogue receptor and is involved in regulating growth hormone release. Obestatin was initially reported to be an endogenous ligand for the orphan G protein-coupled receptor GPR39 and was involved in satiety and decreased food intake; however, these findings are controversial. Recent reports show that obestatin is involved in inhibiting thirst and anxiety, improving memory, regulating sleep, affecting cell proliferation, and increasing the secretion of pancreatic juice enzymes. Alternative promoters and alternative splicing result in multiple transcript variants, some of which encode different protein isoforms and some of which do not encode a protein but may regulate the ghrelin-obestatin preproprotein expression. In addition, antisense transcripts for this gene have been identified and may also function in regulation of the ghrelin-obestatin preproprotein expression. [provided by RefSeq, Oct 2008]. Function: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation. Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility. Subcellular Location: Secreted. Tissue Specificity: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy. Post-translational modifications: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid. Amidation of Leu-98 is essential for obestatin activity. Similarity: Belongs to the motilin family. SWISS: Q9UBU3 Gene ID: 51738 Database links: Entrez Gene: 51738 Human Entrez Gene: 58991 Mouse Entrez Gene: 59301 Rat Entrez Gene: 574280 Rhesus monkey Omim: 605353 Human SwissProt: Q6BEG6 Cat SwissProt: Q9BEF8 Dog SwissProt: Q9UBU3 Human SwissProt: Q9EQX0 Mouse SwissProt: Q9GKY5 Pig SwissProt: Q9QYH7 Rat Unigene: 590080 Human Unigene: 672979 Human Unigene: 379095 Mouse Unigene: 42103 Rat Ghrelin是近年来发现的一种内源性生长激素促分泌剂受体的配体（一种新的生长激素释放肽）。 广泛分布在人中枢神经系统(下丘脑、垂体)和消化系统(胃、肠、胰腺),并被发现拥有多种生物功能,如通过下丘脑刺激食欲、增加胃酸分泌、提高胃肠蠕动等.进而ghrelin和GHS-R在不同器官Tumour组织中被发现,如垂体瘤、胃癌、肠癌、乳腺癌、甲状腺滤泡状癌,Ghrelin蛋白的种属同源性较高，人、大、小鼠、猪、羊、犬、牛都有存在。 大量研究证明ghrelin能影响不同Tumour细胞的生长与增殖.其广泛的生物活性为今后Tumour的诊断、治疗及预后的评估提供了新的研究方向.
Product Picture	Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Ghrelin Polyclonal Antibody, Unconjugated(SL0467R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ghrelin) Polyclonal Antibody, Unconjugated (SL0467R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 7

  7Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• A

  ADelete

• 
• A

  ADelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 4

  4Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout