Product Name	Beta casein
Chinese Name	β-酪蛋白抗体
Alias	Beta-casein; CASB; CASB_SHEEP; Casein beta; CSN 2; Csn2.
literatures	Specific References  (14)     |     SL0466R has been referenced in 14 publications. [IF=6.117] Gang Lin. et al. ARID1B blocks methionine-stimulated mTOR activation to inhibit milk fat and protein synthesis in and proliferation of mouse mammary epithelial cells. J NUTR BIOCHEM. 2023 Jan;:109274  IF ;  Mouse.   PubMed:36681308 [IF=6.048] Hao Qi. et al. Methionine and leucine induce ARID1A degradation to promote mTOR expression and milk synthesis in mammary epithelial cells. J Nutr Biochem. 2021 Nov;:108924  WB,IF ;  Cow.   PubMed:34843932 [IF=5.895] Xinling Wang. et al. Enhancement of BCAT2-Mediated Valine Catabolism Stimulates β-Casein Synthesis via the AMPK-mTOR Signaling Axis in Bovine Mammary Epithelial Cells. J AGR FOOD CHEM. 2022;XXXX(XXX):XXX-XXX  WB, IF ;  Bovine.   PubMed:35916279 [IF=5.561] Xinyang Fan. et al. CEBPA-Regulated Expression of SOCS1 Suppresses Milk Protein Synthesis through mTOR and JAK2-STAT5 Signaling Pathways in Buffalo Mammary Epithelial Cells. FOODS. 2023 Jan;12(4):708  WB ;  Bovine.   PubMed:36832783 [IF=5.396] Zhiyun Hao. et al. MicroRNA-432 inhibits milk fat synthesis by targeting SCD and LPL in ovine mammary epithelial cells. Food Funct. 2021 Aug;:  WB ;  Sheep.   PubMed:10.1039/D1FO01260F [IF=5.223] Xinyang Fan. et al. MiR-190a regulates milk protein biosynthesis through the mTOR and JAK2–STAT5 signaling pathways by targeting PTHLH in buffalo mammary epithelial cells. J FUNCT FOODS. 2023 Mar;102:105451  WB ;  Bovine.   PubMed:10.1016/j.jff.2023.105451 [IF=5.168] Xinling Wang. et al. Plasma Proteomic Profiling Reveals the Regulatory Factors of Milk Protein Synthesis in Holstein Cows. BIOLOGY-BASEL. 2022 Aug;11(8):1239  WB ;  Bovine.   PubMed:36009866 [IF=4.092] Ludmila K et al. Development of Immunohistochemical Methods for Casein Detection in Meat ProductsFoods.2020 Dec 24;10(1):28.  IHC ;  Cow、Pig.   PubMed:33374134 [IF=4.074] Zhang M et al. Proteomic analysis reveals proteins and pathways associated with lactation in bovine mammary epithelial cell-derived exosomes. J Proteome Res . 2020 Aug 7;19(8):3211-3219.  WB ;  Cow.   PubMed:32506918 [IF=3.571] Li P et al. CRTC2 is a key mediator of amino acid-induced milk fat synthesis in mammary epithelial cells. J Agric Food Chem. 2019 Sep 18;67(37):10513-10520.  WB ;  Cow.   PubMed:31475823 [IF=3.333] Jinyu Zhou et al. Prolactin regulates LAT1 expression via STAT5 (signal transducer and activator of transcription 5) signaling in mammary epithelial cells of dairy cows. J Dairy Sci. 2020 Jul;103(7):6627-6634.  WB ;  Cow.   PubMed:32389479 [IF=1.778] Z.Y. Hao. et al. Deep small RNA-Seq reveals microRNAs expression profiles in lactating mammary gland of 2 sheep breeds with different milk performance. Domest Anim Endocrin. 2021 Jan;74:106561  IHC ;  Sheep.   PubMed:33035848 [IF=1.421] Li B et al. Septin6 regulates cell growth and casein synthesis in dairy cow mammary epithelial cells viamTORC1 pathway. J Dairy Res. 2019 May;86(2):181-187.  WB ;  Dairy cow.   PubMed:31122298 [IF=]   IHC ;  ewes.   PubMed:33035848
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Cow, Sheep, Goat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	23kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from sheep Beta-casein: 151-222/222
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Milk proteins are crucial for the development of all newborn mammals and caseins constitute the major proteins in mammalian milk. b- and k-caseins are the only caseins present in human milk. The b-casein/k-casein ratio is higher in colostrum than in transitional and mature milk and is related to a better digestibility of colostrum casein micelles by the neonate during the first days of life. Human b-casein-encoding gene (Bca) contains a highly phosphorylated site, which is responsible for the calcium-binding capacity of b-casein. A common set of transcription factors are required for the expression of b-casein. Multiple binding sites for Stat5, C/EBPb (CCAAT/enchancer-binding protein) and several half-sites for glucocorticoid receptor (GR) are identified in the distal human enhancer of the b-casein gene. b-casein gene transcription is regulated primarily by a composite response element (CoRE), which integrates signaling from the lactogenic hormones PRL, insulin and hydrocortisone in mammary epithelial cells. NFkB functions as a negative regulator of b-casein gene expression during pregnancy by interfering with Stat5 tyrosine phosphorylation. Function: Important role in determination of the surface properties of the casein micelles. Subcellular Location: Secreted. Tissue Specificity: Mammary gland specific. Secreted in milk. Similarity: Belongs to the beta-casein family. SWISS: P11839 Gene ID: 443391 Database links: Entrez Gene: 443391 Sheep Entrez Gene: 1447 Human Omim: 115460 Human SwissProt: P05814 Human Unigene: 2242 Human β-酪蛋白常在乳腺表达，山羊、绵羊同源 分子量：25kDa
Product Picture	Sample: Lane 1: Milk (Goat) Lysate at 2 ug Lane 2: Milk (Cow) Lysate at 30 ug Lane 3: Milk (Cow) Lysate at 3 ug Lane 4: Milk (Human) Lysate at 30 ug Primary: Anti-Beta-casein (SL0466R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 25-29 kD Observed band size: 25-29 kD

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