Product Name	MMP1
Chinese Name	基质金属蛋白酶-1抗体
Alias	27 kDa interstitial collagenase; CLGN; CLG; collagenase, fibroblast; Fibroblast collagenase; Interstitial collagenase; MMP 1; MMP-1; MMP; Fibroblast collagenase; Interstitial collagenase; Matrix metallopeptidase 1 (interstitial collagenase); Matrix metalloprotease 1; Matrix metalloproteinase-1; MMP1_HUMAN; OTTHUMP00000045866; Matrix Metalloproteinase 1.
literatures	Specific References  (12)     |     SL0424R has been referenced in 12 publications. [IF=6.656] Yangxin Li. et al. Ershiwuwei Lvxue Pill alleviates rheumatoid arthritis by different pathways and produces changes in the gut microbiota. PHYTOMEDICINE. 2022 Dec;107:154462  IHC ;  Rat.   PubMed:36162242 [IF=6.639] Hyeon Woo Kim. et al. Matrix Metalloproteinase-1 (MMP1) Upregulation through Promoter Hypomethylation Enhances Tamoxifen Resistance in Breast Cancer. Cancers. 2022 Jan;14(5):1232  WB,IHC ;  Human,Mouse.   PubMed:35267540 [IF=5.5] Lu Z et al. A Novel Synthesized Sulfonamido-Based Gallate-JEZTC Blocks Cartilage Degradation on Rabbit Model of Osteoarthritis: An in Vitro and in Vivo Study.(2018) Cell Physiol Biochem.49(6):2304-2319  IHC ;  Rabbit.   PubMed:30261492 [IF=5.195] Yong Wang. et al. An integrated network pharmacology approach reveals that Darutigenol reduces inflammation and cartilage degradation in a mouse collagen-induced arthritis model by inhibiting the JAK-STAT3 pathway. J ETHNOPHARMACOL. 2023 May;:116574  WB ;  Mouse.   PubMed:37160212 [IF=2.766] Li et al. Visfatin Destabilizes Atherosclerotic Plaques in Apolipoprotein E-Deficient Mice. (2016) PLoS.One. 11:e0148273  WB ;  Rat.   PubMed:26848572 [IF=2.605] Wang Yaxin. et al. Overexpression of estrogen receptor β inhibits cellular functions of human hepatic stellate cells and promotes the anti-fibrosis effect of calycosin via inhibiting STAT3 phosphorylation. BMC PHARMACOL TOXICO. 2022 Dec;23(1):1-13  WB ;  Human.   PubMed:36207725 [IF=2.535] Lei J et al. LncRNA SNHG1 Alleviates IL-1β-induced Osteoarthritis by Inhibiting miR-16-5p-mediated p38 MAPK and NF-κB Signaling Pathways. Biosci Rep. 2019 Aug 5. pii: BSR20191523.  WB ;  Human.   PubMed:31383786 [IF=2.466] Jinjiang Yang. et al. Platelet-rich plasma attenuates interleukin-1β-induced apoptosis and inflammation in chondrocytes through targeting hypoxia-inducible factor-2α. Tissue Cell. 2021 Sep;:101646  WB ;  rat.   PubMed:34536814 [IF=2.14] Lu, Minling, et al. "The effects of mycotoxins and selenium deficiency on tissue-engineered cartilage." Cells Tissues Organs 196.3 (2012): 241-250.  IHC-P ;  Rabbit.   PubMed:22538829 [IF=2.05] Zhang M et al. Hepatoprotective effect and possible mechanism of phytoestrogen calycosin on carbon tetrachloride–induced liver fibrosis in mice. Naunyn Schmiedebergs Arch Pharmacol . 2020 May 30.  WB ;  Mouse.   PubMed:32474674 [IF=2.05] Mengmeng Zhang. et al. Hepatoprotective effect and possible mechanism of phytoestrogen calycosin on carbon tetrachloride–induced liver fibrosis in mice. N-S Arch Pharmacol. 2021 Jan;394(1):189-204  WB ;  Mouse.   PubMed:32474674 [IF=1.55] Yang, Jinjiang, Ying Lu, and Ai Guo. "Platelet-rich plasma protects rat chondrocytes from interleukin-1β-induced apoptosis." Molecular Medicine Reports 14.5 (2016): 4075-4082.  WB ;  Rat.   PubMed:27665780
Research Area	Tumour  Cardiovascular  Cell biology  Signal transduction  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rabbit,  (predicted: Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	27/41/54kDa
Cellular localization	Extracellular matrix Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MMP1: 401-469/469
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Mar 2009] Function: Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. Subunit: Interacts with HIV-1 Tat. Subcellular Location: Secreted, extracellular space, extracellular matrix (Probable). Post-translational modifications: Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains. SWISS: P03956 Gene ID: 4312 Database links: Entrez Gene: 281308 Cow Entrez Gene: 4312 Human Entrez Gene: 17386 Mouse Entrez Gene: 397320 Pig Entrez Gene: 100009110 Rabbit Omim: 120353 Human SwissProt: P28053 Cow SwissProt: P03956 Human SwissProt: P33435 Mouse SwissProt: P21692 Pig SwissProt: P13943 Rabbit Unigene: 83169 Human Synthesis and Degradation（Synthesis and Degradation） 基质金属蛋白酶(matrix metalloproteinases, MMPs)是一族依赖锌离子而降解各种Extracellular matrix的蛋白酶，亦称IV型胶原酶或称明胶酶A，其主要功能为降解IV型胶原，因而它在Tumour细胞突破基底膜屏障和浸润转移中起重要作用。 目前主要用于各种恶性Tumour(如乳腺癌、胃肠道癌、卵巢癌、膀胱癌等)中的基底膜检测与Tumour转移浸润的研究。
Product Picture	Sample: Placenta (Mouse) Lysate at 30 ug Primary: Anti- MMP-1 (SL0424R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27 kD Observed band size: 27 kD Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-MMP-1 (SL0424R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 27/54kD Observed band size: 50kD Sample: Heart (Rabbit) Lysate at 30 ug Primary: Anti-MMP-1 (SL0424R) at 1:300; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1: 5000; Predicted band size: 24 kD Observed band size: 27 kD Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MMP-1 Polyclonal Antibody, Unconjugated(SL0424R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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