Product Name	TIMP-1
Chinese Name	金属蛋白酶组织抑制因子-1抗体
Alias	Clgi; Collagenase inhibitor; EPA; EPO; Erythroid Potentiating Activity; Fibroblast collagenase inhibitor; FLJ90373; HC; Human Collagenase Inhibitor; Metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1 precursor; OTTHUMP00000023214; TIMP 1; TIMP; TIMP metallopeptidase inhibitor 1; TIMP1 protein; Tissue Inhibitor of Metalloproteinase 1; Tissue inhibitor of metalloproteinases; Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor.
literatures	Specific References  (26)     |     SL0415R has been referenced in 26 publications. [IF=6.543] Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022  WB ;  Rat,Human.   PubMed:35308169 [IF=5.223] Choirul Anwar. et al. Molecular mechanisms of Agardhiella subulata attenuates hepatic fibrosis by modulating hepatic stellate cell activation via the reduction of autophagy. J FUNCT FOODS. 2022 Sep;96:105226  WB ;  Rat.   PubMed:10.1016/j.jff.2022.105226 [IF=5.064] Xia Liu. et al. The adipokine orosomucoid alleviates adipose tissue fibrosis via the AMPK pathway. Acta Pharmacol Sin. 2021 Apr;:1-9  WB ;  Mouse.   PubMed:33875797 [IF=4.486] Xu T et al. MicroRNA‐708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med . 2020 Jul;24(13):7127-7140.  WB ;  Human.   PubMed:32463570 [IF=4.12] Li et al. Neurogenic differentiation factor NeuroD confers protection against radiation-induced intestinal injury in mice. (2016) Sci.Rep. 6:30180  IHC ;  Rat.   PubMed:27436572 [IF=4.081] Guo, Ziwei. et al. Single and Combined Effects of Short-Term Selenium Deficiency and T-2 Toxin-Induced Kidney Pathological Injury Through the MMPs/TIMPs System. BIOL TRACE ELEM RES. 2023 Jan;:1-11  IHC ;  Rat.   PubMed:36645617 [IF=3.56] Sassoli, Chiara, et al. "Defining the role of mesenchymal stromal cells on the regulation of matrix metalloproteinases in skeletal muscle cells." Experimental Cell Research (2014).  Mouse.   PubMed:24631289 [IF=3.53] Lu HY, Huang CY, Shih CM, Chang WH, Tsai CS, et al. (2015) Dipeptidyl Peptidase-4 Inhibitor Decreases Abdominal Aortic Aneurysm Formation through GLP-1-Dependent Monocytic Activity in Mice. PLoS ONE 10(4): e0121077.  IHC-P ;  Mouse.   PubMed:25876091 [IF=3.361] Varghese S et al. Immunostimulatory plant polysaccharides impede cancer progression and metastasis by avoiding off-target effects. Int Immunopharmacol. 2019 May 21;73:280-292.  WB ;  Human.   PubMed:31125927 [IF=3.31] Watts, Ryan P., et al. "Novel 24-h ovine model of brain death to study the profile of the endothelin axis during cardiopulmonary injury." Intensive Care Medicine Experimental 3.1 (2015): 31.  IHC-P ;   PubMed:26596583 [IF=3.204] Chi Li. et al. Schisantherin D from Schisandra chinensis (Turcz.) Baill. exhibits anti-liver fibrosis capacity via modulating ETBR involved signaling, an in vitro and in vivo study. FITOTERAPIA. 2022 Oct;162:105290  WB ;  Mouse, Human.   PubMed:36064152 [IF=3.157] Liu et al. Hydrogen coadministration slows the development of COPD-like lung disease in a cigarette smoke-induced rat model. (2017) Int.J.Chron.Obstruct.Pulmon.Di. 12:1309-1324  IHC-P,WB ;  Rat.   PubMed:28496315 [IF=3.14] Varghese, Sheeja, et al. "The inhibitory effect of anti-tumor polysaccharide from Punica granatum on metastasis." International Journal of Biological Macromolecules (2017).  WB ;  Human.   PubMed:28552725 [IF=3.082] Xiaolan Ouyang. et al. Transplantation of Human Amnion Epithelial Cells Improves Endometrial Regeneration in Rat Model of Intrauterine Adhesions. Stem Cells Dev. 2020 Oct;29(20):1346-1362  IHC ;  Human.   PubMed:32772798 [IF=2.745] linlin zeng. et al. Atractylodes Processing Products Protect against Gastric Ulcers in Rats by Influencing the NF-κB-MMP-9/TIMP-1 Regulatory Mechanism and Intestinal Flora. CHEM BIODIVERS. 2023 Apr;:e202300068  WB ;  Rat.   PubMed:37102636 [IF=2.705] Wang EX et al. Stem cells from trabecular meshwork cells can secrete extracellular matrix. Biochem Biophys Res Commun. 2020 Mar 5;523(2):522-526.  ICF ;  cattle.   PubMed:31902587 [IF=2.62] Sassoli, Chiara, et al. "Low intensity 635 nm diode laser irradiation inhibits fibroblast–myofibroblast transition reducing TRPC1 channel expression/activity: New perspectives for tissue fibrosis treatment." Lasers in Surgery and Medicine (2016).  Mouse.   PubMed:26660509 [IF=2.53] Ni, Wei-Jian, et al. "Renoprotective effects of berberine through regulation of the MMPs/TIMPs system in streptozocin-induced diabetic nephropathy in rats."European Journal of Pharmacology (2015).  WB ;  Rat.   PubMed:26192633 [IF=2.408] Gundogdu, Gokhan. et al. Characterization of a novel rabbit model of Peyronie’s disease. INT J IMPOT RES. 2023 Feb;:1-6  IHC ;  Rabbit.   PubMed:36782023 [IF=2.362] Shi, Bohui. et al. Polymorphism of MMP-3 gene and imbalance expression of MMP-3 / TIMP-1 in articular cartilage are associated with an endemic osteochondropathy, Kashin- Beck disease. Bmc Musculoskel Dis. 2022 Dec;23(1):1-7  IHC ;  Human.   PubMed:34980041 [IF=2.362] Zhao R et al. Interleukin-1 receptor antagonist protein (IL-1Ra) and miR-140 overexpression via pNNS-conjugated chitosan-mediated gene transfer enhances the repair of full-thickness cartilage defects in a rabbit model. Bone Joint Res. 2019 Apr 2;8(3):165-178.  WB ;  Human.   PubMed:30997042 [IF=2.25] Shi S et al. MicroRNA-34a attenuates VEGF-mediated retinal angiogenesis via targeting the Notch1.(2018) Biochemistry and Cell Biology  WB ;  Rat &Human.   PubMed:30571142 [IF=2.247] Tort S et al. The effect of a new wound dressing on wound healing: Biochemical and histopathological evaluation. Burns. 2019 Dec 17. pii: S0305-4179(18)30976-8.  IHC-P ;  Rat.   PubMed:31862280 [IF=2.14] Lu, Minling, et al. "The effects of mycotoxins and selenium deficiency on tissue-engineered cartilage." Cells Tissues Organs 196.3 (2012): 241-250.  IHC-P ;  Rabbit.   PubMed:22538829 [IF=2.05] Mengmeng Zhang. et al. Hepatoprotective effect and possible mechanism of phytoestrogen calycosin on carbon tetrachloride–induced liver fibrosis in mice. N-S Arch Pharmacol. 2021 Jan;394(1):189-204  WB ;  Mouse.   PubMed:32474674 [IF=1.26] Jiang, Li, Tongfu Zhou, and Hanming Liu. "Combined effects of the ATP-sensitive potassium channel opener pinacidil and simvastatin on pulmonary vascular remodeling in rats with monocrotaline-induced pulmonary arterial hypertension." Die Pharmazie-An International Journal of Pharmaceutical Sciences 67.6 (2012): 547-552.  IHC-P ;  Rat.   PubMed:22822545
Research Area	Tumour  Cell biology  Neurobiology  Apoptosis  Growth factors and hormones  Kinases and Phosphatases  Synthesis and Degradation
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rabbit,  (predicted: Rat, Dog, Pig, Cow, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	21kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TIMP-1: 103-207/207
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq]. Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Subcellular Location: Secreted. Post-translational modifications: The activity of TIMP1 is dependent on the presence of disulfide bonds. Similarity: Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain. SWISS: P01033 Gene ID: 7076 Database links: Entrez Gene: 7076 Human Entrez Gene: 21857 Mouse Omim: 305370 Human SwissProt: P01033 Human SwissProt: P12032 Mouse Unigene: 522632 Human Unigene: 8245 Mouse Synthesis and Degradation（Synthesis and Degradation） TIMP1是基质金属蛋白酶的抑制酶，其功能与MMP相反，能抑制Tumour细胞的浸润和转移，主要用于各种恶性Tumour如乳腺癌等的研究。TIMP-1是一种28kDa的glycoprotein，主要分泌细胞为多种结缔组织细胞，被认为是组织中MMPs活性的主要调节者，它可以和MMPs家族成员以共价键方式形成1:1的复合物，从而发挥其抑制作用。在正常生理条件下，MMPs和TIMPs之间的平衡状态是维持Extracellular matrix正常状态所必需的，一旦它们之间的比例失调，就会造成一系列的病理状态。
Product Picture	Sample: Lane 1: TIMP-1 fusion protein (SL43009P) 0.2ug Primary: Anti-TIMP-1(SL0415R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21kDa Observed band size: 25kDa Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated(SL0415R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated(SL0415R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated(SL0415R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rabbit liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TIMP-1 Polyclonal Antibody, Unconjugated(SL0415R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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