Product Name	VCAM1
Chinese Name	vascular endothelial cell粘附分子（CD106）抗体
Alias	VCAM1; CD 106; CD106; CD106 Antigen; DKFZp779G2333; INCAM 100; L1CAM; MGC99561; Vascular Cell Adhesion Molecule 1; Vascular cell adhesion protein 1; VCAM 1; VCAM-1; INCAM-100; V-CAM 1; VCAM1_HUMAN.
literatures	Specific References  (16)     |     SL0396R has been referenced in 16 publications. [IF=16.834] Moccetti F et al. Myocardial Infarction Produces Sustained Proinflammatory Endothelial Activation in Remote Arteries. J Am Coll Cardiol. 2018 Aug 28;72(9):1015-1026.  IHF ;  Mouse.   PubMed:30139430 [IF=10.334] Xin-Sen Chen. et al. Losartan attenuates sepsis-induced cardiomyopathy by regulating macrophage polarization via TLR4-mediated NF-κB and MAPK signaling. PHARMACOL RES. 2022 Nov;185:106473  WB ;  Mouse.   PubMed:36182039 [IF=8.786] Guangxu Xiao. et al. CXCR1 and its downstream NF-κB inflammation signaling pathway as a key target of Guanxinning injection for myocardial ischemia/reperfusion injury.. FRONT IMMUNOL. 2022 Oct;13:1007341-1007341  WB, IF ;  Mouse.   PubMed:36325326 [IF=5.646] Ye T et al. MicroRNA-7 as a potential therapeutic target for aberrant NF-κB-driven distant metastasis of gastric cancer. J Exp Clin Cancer Res. 2019 Feb 6;38(1):55.  FCM&IHC ;  Mouse&Human.   PubMed:30728051 [IF=5.235] Pu, Luya. et al. Laminar shear stress alleviates monocyte adhesion and atherosclerosis development via miR-29b-3p/CX3CL1 axis regulation. J CELL SCI. 2022 Jul;135(14):  WB ;  Human.   PubMed:35735031 [IF=5.151] Zhang L et al. The Protective Activities of Dietary Sea Cucumber Cerebrosides against Atherosclerosis through Regulating Inflammation and Cholesterol Metabolism in Male Mice.Mol Nutr Food Res. 2018 Aug;62(16):e1800315.  WB ;  Mouse.   PubMed:29883529 [IF=4.932] Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFκB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096  WB ;  Human.   PubMed:34464886 [IF=4.545] Chae Eun Kim. et al. Cevimeline-induced anti-inflammatory effect through upregulations of mucins in the ocular surface of a dry eye mouse model. Biomed Pharmacother. 2021 Jul;139:111571  IHC ;  Mouse.   PubMed:33857915 [IF=3.97] Ding, Lin, et al. "Eicosapentaenoic acid-enriched phospholipids improve atherosclerosis by mediating cholesterol metabolism." Journal of Functional Foods 32 (2017): 90-97.  WB ;  Mouse.   PubMed:doi:10.1016/j.jff.2017.02.020 [IF=3.968] Wei Tang. et al. Macrophage membrane-mediated targeted drug delivery for treatment of spinal cord injury regardless of the macrophage polarization states. Asian J Pharm Sci. 2021 Apr;:  WB ;  Human.   PubMed:10.1016/j.ajps.2021.03.005 [IF=3.905] Fen Xu. et al. Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway. Exp Cell Res. 2022 Mar;412:113025  IF ;  Rat.   PubMed:35026282 [IF=3.47] Ding L et al. Saponins of sea cucumber attenuate atherosclerosis in ApoE −/− mice via lipid-lowering and anti-inflammatory properties. Journal of Functional Foods,2018 48, 490–497.  WB ;  Rat&Mouse.   PubMed:10.1016/j.jff.2018.07.046 [IF=3.241] Zhang L et al. Eicosapentaenoic acid in the form of phospholipid exerts superior anti-atherosclerosis effects than its triglyceride form in ApoE-/-mice. Food & Function. 2019  WB ;  Mouse.   PubMed:31246210 [IF=2.976] Peng Y et al. Sonodynamic therapy improves anti‑tumor immune effect by increasing the infiltration of CD8+ T cells and altering tumor blood vessels in murine B16F10 melanoma xenograft. Oncol Rep. 2018 Oct;40(4):2163-2170.   IHF ;  Mouse.   PubMed:30106435 [IF=2.586] Yunan Wang. et al. A novel type of mesenchymal stem cells derived from bovine metanephric mesenchyme. TISSUE CELL. 2022 Dec;79:101970  IF ;  Bovine.   PubMed:36370625 [IF=2.314] Ma H et al. Interferon-γ facilitated adjuvant-induced arthritis at early stage. Scand J Immunol. 2019 Feb 9:e12757.  WB ;  Mouse.   PubMed:30739356
Research Area	Tumour  Cardiovascular  Cell biology  Cell adhesion molecule  endothelial cells
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1µg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	81kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse VCAM-1: 640-739/739 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	VCAM1 is important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the integrins alpha4 beta1 (beta 1 integrin VLA4) and alpha4 beta7 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. VCAM1 is also expressed by several non endothelial cell types including some macrophages, follicular dendritic cells and bone marrow, stromal cells. Function: Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. Subunit: Binds to ECMV-D capsid proteins and acts as a receptor for this virus. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. Expressed in the bone marrow. Similarity: Contains 7 Ig-like C2-type (immunoglobulin-like) domains. SWISS: P29533 Gene ID: 22329 Database links: Entrez Gene: 7412 Human Entrez Gene: 22329 Mouse Entrez Gene: 25361 Rat GenBank: NP_001069.1 Human GenBank: NP_001186763.1 Human Omim: 192225 Human SwissProt: P19320 Human SwissProt: P29533 Mouse VCAM 1（CD106）是一种膜相关蛋白，分布于血管内皮及其周围的lymphocyte、单核细胞、嗜酸性粒细胞和中性粒细胞。Anti-VCAM-1主要用于各种组织中血管内皮的检测。
Product Picture	Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Spleen tissue lysates Lane 3: Mouse Cerebrum tissue lysates Primary: Anti-VCAM1 (SL0396R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kDa Observed band size: 100 kDa Sample: Lane 1: Siha (Human) Cell Lysate at 30 ug Lane 2: Huvec (Human) Cell Lysate at 30 ug Lane 3: U251 (Human) Cell Lysate at 30 ug Primary: Anti-VCAM-1 (SL0396R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 110 kD Observed band size: 110 kD Sample: VCAM1 protein (Human) at 100 ng Primary: Anti-VCAM-1 (SL0396R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 81 kD Observed band size: 81 kD Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-VCAM-1 Polyclonal Antibody, Unconjugated(SL0396R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: Mouse Spleen(blue). Primary Antibody:Rabbit Anti-VCAM-1 antibody (SL0396R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (SL0396R, 1μg /8x10^5 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (1 hour) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.

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