Product Name	Thymosin Alpha-1
Chinese Name	胸腺肽α1抗体
Alias	Prothymosin alpha (gene sequence 28); Prothymosin alpha; Prothymosin alpha like 1 protein; Thymalfasin; Thymosin alpha 1 acetate; Thymosin alpha 1; Thymosin alpha; TMSA; PTMA; PTMA_HUMAN.
literatures	Specific References  (1)     |     SL0226R has been referenced in 1 publications. [IF=2.42] Shao, Congwen, et al. "Thymosin alpha-1-transformed< i> Bifidobacterium promotes T cell proliferation and maturation in mice by oral administration." International immunopharmacology (2013).  WB ;   PubMed:23352493
Research Area	Tumour  immunology  Endocrinopathy
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Cow, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	12kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Thymosin Alpha-1: 2-29/111
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The thymus gland produces several hormones or hormone-like substances which are derived from a polypeptide precursor containing (in the rat) 113 amino acids and known as prothymosin alpha. A peptide containing 28 amino acid residues, named thymosin alpha 1, was originally isolated from calf thymosin fraction 5 and shown to restore various aspects of immune function in several in vitro and in vivo test systems. Thymosin alpha 1 was subsequently isolated from a similar fraction from human thymosin and reported to have the same amino acid sequence as bovine thymosin alpha 1. Function: Prothymosin alpha may mediate immune function by conferring resistance to certain opportunistic infections. Subcellular Location: Nucleus. Post-translational modifications: Covalently linked to a small RNA of about 20 nucleotides. Similarity: Belongs to the pro/parathymosin family. SWISS: P06454 Gene ID: 5757 Database links: Entrez Gene: 5757 Human Omim: 188390 Human SwissProt: P06454 Human Unigene: 459927 Human Unigene: 708083 Human 胸腺素α-1；胸腺肽
Product Picture	MCF7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Thymosin Alpha-1) polyclonal Antibody, Unconjugated (SL0026R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control: H9C2 Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-PE-CY5, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5μl in 100 μL1X PBS containing 0.5% BSA. Blank control: RSC96 Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-APC, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 2μl in 100 μL1X PBS containing 0.5% BSA. The blue histogram is unstained cells(293FT cells). The Wathet Blue histogram is cells stained with secondary antibody alone. The Orange histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody. The green histogram is cells stained withRabbit Anti-Thymosin Alpha-1 antibody (SL0226R) plus secondary antibody. Concentration 1:50

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