Product Name	CEA
Chinese Name	癌胚抗原抗体
Alias	Carcinoembryonic antigen; Carcinoembryonic antigen related cell adhesion molecule 5; Carcinoembryonic antigen-related cell adhesion molecule 5; CD66e; CD66e; CD66e antigen; CEACAM5; CEAM5_HUMAN; DKFZp781M2392; Meconium antigen 100.
literatures	Specific References  (4)     |     SL0060R has been referenced in 4 publications. [IF=6.057] Limin Zhou. et al. Copper doped terbium metal organic framework as emitter for sensitive electrochemiluminescence detection of CYFRA 21-1. Talanta. 2022 Feb;238:123047   PubMed:34801904 [IF=2.678] Li J et al. A highly specific and sensitive fluorescence quenching probe for carcinoembryonic antigen detection based on tetrapod Au nanostars with Ag coating. Materials Today Communications (2020).  Other ;   PubMed:10.1016/j.mtcomm.2020.101373 [IF=2.678] Jian-Jun Liet al. A highly specific and sensitive fluorescence quenching probe for carcinoembryonic antigen detection based on tetrapod Au nanostars with Ag coating. Materials Today Communications, 101373.  other ;   PubMed:10.1016/j.mtcomm.2020.101373 [IF=0] Chen L et al. Local extraction and detection of early stage breast cancers through a microneedle and nano-Ag/MBL film based painless and blood-free strategy. Materials Science and Engineering: C,2019. 110402.  Other ;   PubMed:doi:10.1016/j.msec.2019.110402
Research Area	Tumour  immunology  Stem cells  TumourCell biologyMaker
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	71kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CEA: 51-120/349
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	CEA-related cell adhesion molecules (CEACAM) belong to the carcinoembryonic antigen (CEA) family. It consists of seven CEACAM (CEACAM 1, CEACAM 3-CEACAM 8) and 11 pregnancy-specific glyco-protein (PSG 1-PSG 11) members. The CEA family proteins belong to the immunoglobulin (Ig) superfamily and are composed of one Ig variable-like (IgV) and a varying number (0-6) of Ig constant-like (IgC) domains. CEACAM molecules are membrane-bound either via a transmembrane domain or a glycosyl phosphatidyl inositol (GPI) anchor. CEACAM molecules are differentially expressed in epithelial cells or in leucocytes. Over-expression of CEA/ CEACAM 5 in tumors of epithelial origin is the basis of its wide-spread use as a tumor marker. The function of CEACAM family members varies widely: they function as cell adhesion molecules, tumor suppressors, regulators of lymphocyte and dendritic cell activation, receptors of Neisseria species and other bacteria. Function: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Subunit: Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor Tissue Specificity: Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Post-translational modifications: Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate. Similarity: Belongs to the immunoglobulin superfamily. CEA family. Contains 7 Ig-like (immunoglobulin-like) domains. SWISS: P40199 Gene ID: 4680 Database links: Entrez Gene: 4680 Human Entrez Gene: 100125369 Rat Omim: 163980 Human SwissProt: P40199 Human Unigene: 466814 Human Unigene: 122781 Rat CEA是一种胚胎性抗原，主要存在于胎儿消化道上皮组织，胰腺和肝癌中。CEA广泛存在各种上皮性Tumour，尤其是胃肠道恶性Tumour，CEA分布阳性类型与Tumour的恶性度有关。CEA与EMA一样可作为上皮性恶性Tumour的重要标记。
Product Picture	Sample: SW480 Cell Lysate at 40 ug Primary: Anti- CEA(SL0060R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 71 kD Observed band size: 71 kD Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADRAIA) Polyclonal Antibody, Unconjugated (SL0060R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CEA/CD66e/CEACAM5 Polyclonal Antibody, Unconjugated(SL0060R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (blue line): MCF7 (blue). Primary Antibody (green line): Rabbit Anti-CEA antibody (SL0060R) Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% methanol overnight at 4℃ . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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