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Product Name Anti-LMP2/AF594 Chinese Name AF594标记的低分子质量蛋白2抗体 Alias Proteasome 20S LMP2; Large multifunctional peptidase 2; LMP 2; LMP2; Low molecular mass protein 2; Macropain chain 7; MGC70470; Multicatalytic endopeptidase complex chain 7; Proteasome beta 9 subunit; Proteasome catalytic subunit 1i; Proteasome chain 7; Proteasome related gene 2; Proteasome subunit beta 6i; Proteasome subunit beta type 9; PSMB 9; PSMB9; PSB9_HUMAN. Research Area Cell biology immunology Immunogen Species Rabbit Clonality Polyclonal React Species Applications ICC=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 23kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Proteasome 20S LMP2 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 1 (proteasome beta 6 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. [provided by RefSeq, Mar 2010]
Function:
The proteasome is a multicatalytic proteinase complexwhich is characterized by its ability to cleave peptides with Arg,Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral orslightly basic pH. The proteasome has an ATP-dependent proteolyticactivity. This subunit is involved in antigen processing togenerate class I binding peptides. Replacement of PSMB6 by PSMB9increases the capacity of the immunoproteasome to cleave modelpeptides after hydrophobic and basic residues.
Subunit:
The 26S proteasome consists of a 20S proteasome core andtwo 19S regulatory subunits. The 20S proteasome core is composed of28 subunits that are arranged in four stacked rings, resulting in abarrel-shaped structure. The two end rings are each formed by sevenalpha subunits, and the two central rings are each formed by sevenbeta subunits. The catalytic chamber with the active sites is onthe inside of the barrel. This subunit is part of theimmunoproteasome where it displaces the equivalent houskeepingsubunit PSMB6. Interacts with HIV-1 TAT protein.
Subcellular Location:
Cytoplasm. Nucleus.
Tissue Specificity:
Highly expressed in immature dendritic cells (at protein level).
Post-translational modifications:
Autocleaved. The resulting N-terminal Thr residue of themature subunit is responsible for the nucleophile proteolyticactivity.
Similarity:
Belongs to the peptidase T1B family.
Database links:
Entrez Gene: 5698 Human
Entrez Gene: 16912 Mouse
Omim: 177045 Human
SwissProt: P28065 Human
SwissProt: P28076 Mouse
Unigene: 654585 Human
Unigene: 390983 Mouse
Unigene: 13686 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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