Product Name	SF3B3
Chinese Name	选择性剪接因子3B亚基3Recombinant rabbit monoclonal anti
Alias	KIAA0017; Pre mRNA splicing factor SF3b 130 kDa subunit; Pre-mRNA-splicing factor SF3b 130 kDa subunit; RSE1; SAP 130; SAP130; SF3b130; SF3B3_HUMAN; Spliceosome associated protein 130; Spliceosome-associated protein 130; Splicing factor 3b subunit 3 130kD; Splicing factor 3B subunit 3;
Research Area	Signal transduction
Immunogen Species	Rabbit
Clonality	Monoclonal
Clone NO.	R7F1
React Species	(predicted: Human, Mouse, Rat, )
Applications	WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-1:400 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	136kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SF3B3
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes may function in chromatin modification, transcription, splicing, and DNA repair. [provided by RefSeq, Jul 2008] Subcellular Location: Nucleus. SWISS: Q15393 Gene ID: 23450 Database links: Entrez Gene: 23450 Human Entrez Gene: 101943 Mouse Entrez Gene: 292019 Rat SwissProt: Q15393 Human SwissProt: Q921M3 Mouse
Product Picture	Blocking buffer: 5% NFDM/TBST Primary Ab dilution: 1:1000 Primary Ab incubation condition: 2 hours at room temperature Secondary Ab: Goat Anti-Rabbit IgG H&L (HRP) Lysate: 1: HeLa, 2: Jurkat, 3: HEK-293, 4: RAW 264.7, 5: C6 Protein loading quantity: 20 μg Exposure time: 60 s Predicted MW: 135 kDa Observed MW: 135 kDa Tissue: Mouse colon Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:2000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60658R Tissue: Rat cerebrum Section type: Formalin-fixed & Paraffin embedded section Retrieval method: High temperature and high pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary Ab dilution: 1:1000 Primary Ab incubation condition: 1 hour at room temperature Secondary Ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Counter stain: Hematoxylin (Blue) Comment: Color brown is the positive signal for SLM-60658R

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