Product Name	COX4I1(Mitochondrial Loading Control)
Chinese Name	细胞色素c氧化酶IV亚型1（内参）单克隆抗体
Alias	COX41_HUMAN; Cytochrome c oxidase subunit 4 isoform 1, mitochondrial; COX4; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1 (COX IV-1); COX-IV; COX IV; COX 4; COX 4I1;  Mitochondrion内参;
literatures	Specific References  (3)     |     SLM-33037M has been referenced in 3 publications. [IF=13.934] Han Yan. et al. FAM3A maintains metabolic homeostasis by interacting with F1-ATP synthase to regulate the activity and assembly of ATP synthase. METABOLISM. 2022 Dec;:155372  WB ;  Mouse, Human.   PubMed:36470472 [IF=9.685] Chen, Ying. et al. Elevated SFXN2 limits mitochondrial autophagy and increases iron-mediated energy production to promote multiple myeloma cell proliferation. CELL DEATH DIS. 2022 Sep;13(9):1-14  IF ;  Human.   PubMed:36163342 [IF=3.585]    PubMed:32417439
Product Type	Internal reference anti
Research Area	Cell biology  immunology  Mitochondrion
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	8D8
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:200-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	17kDa
Cellular localization	cytoplasmic The cell membrane Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	Recombinant human COX4
Lsotype	IgG
Purification	affinity purified by Protein G
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008] Function: This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Subcellular Location: Mitochondrion inner membrane. Tissue Specificity: Ubiquitous. Similarity: Belongs to the cytochrome c oxidase IV family. SWISS: P13073 Gene ID: 1327 Database links: Entrez Gene: 1327 Human Entrez Gene: 12857 Mouse Entrez Gene: 29445 Rat SwissProt: P13073 Human SwissProt: P19783 Mouse SwissProt: P10888 Rat 细胞色素c氧化酶4亚型1抗体；（细胞色素c氧化酶IV亚型1）是一种存在Mitochondrion上的大型Transmembrane protein复合物。由于细胞色素氧化酶是呼吸电子传递链的第四个中心酶复合物，因此又被称为复合物IV。
Product Picture	Sample: HepG2 Cell (Human) Lysate at 40 ug Primary: Anti- COX4 (SLM-33037M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 17 kD Observed band size: 16 kD Sample: Lane 1: Mouse NIH/3T3 cell lysates Lane 2: Mouse Cerebrum tissue lysates Lane 3: Mouse Kidney tissue lysates Lane 4: Mouse Heart tissue lysates Lane 5: Rat Cerebrum tissue lysates Lane 6: Rat Kidney tissue lysates Lane 7: Rat Heart tissue lysates Lane 8: Human HeLa cell lysates Lane 9: Human Jurkat cell lysates Lane 10: Human Raji cell lysates Lane 11: Human 293T cell lysates Lane 12: Human MCF-7 cell lysates Primary: Anti-COX4I1(Mitochondrial Loading Control) (SLM-33037M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 17 kDa Observed band size: 17 kDa Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (SLM-33037M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (SLM-33037M-8D8) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (SLM-33037M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (SL0296G-CY3) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (Rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COX4) Monoclonal Antibody, Unconjugated (SLM-33037M) at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Mouse IgG antibody (SL0296G-FITC) for 90 minutes, and DAPI for nuclei staining.

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