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Rabbit Anti-PISD antibody
Rabbit Anti-PISD antibody
DJ858B16; dJ858B16.2; DKFZp566G2246; Phosphatidylserine decarboxylase; Phosphatidylserine decarboxylase beta chain; Phosphatidylserine decarboxylase proenzyme; PISD; PISD_HUMAN; PSD; PSDC; PSSC.
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  • NO.:SL8866R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Human,Mouse,(predicted: Rat,Chicken,Dog,Pig,Horse,Rabbit,)
    Applications:WB ELISA IHC-P IHC-F ICC IF
    concentration:1mg/ml
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Details

Product Name PISD
Chinese Name 磷脂酰丝氨酸脱羧酶PISD抗体
Alias DJ858B16; dJ858B16.2; DKFZp566G2246; Phosphatidylserine decarboxylase; Phosphatidylserine decarboxylase beta chain; Phosphatidylserine decarboxylase proenzyme; PISD; PISD_HUMAN; PSD; PSDC; PSSC.  
Research Area Tumour  Cell biology  Signal transduction  The new supersedes the old  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Human, Mouse,  (predicted: Rat, Chicken, Dog, Pig, Horse, Rabbit, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 47kDa
Cellular localization cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human PISD: 151-250/409 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail Enzymes known as phosphatidylserine decarboxylases (PSDs) catalyze the formation of phosphatidylethanolamine from phosphatidylserine via phosphatidylserine decarboxylation. Type I PSDs contain LGST motifs and are found in bacteria and eukaryotic mitochondria, whereas type II PSDs contain GGST motifs and are found in eukaryotic endomembrane systems. PISD (phosphatidylserine decarboxylase), also known as phosphatidylserine decarboxylase proenzyme, PSDC, PSD, PSSC, DJ858B16, dJ858B16.2 or DKFZp566G2246, is a 408 amino acid a type I phosphatidylserine decarboxylase that localizes to the inner mitochondrial membrane. PISD contains a conserved LGST motif which is cleaved to produce two isoforms known as PISD α and PISD β. PISD is capable of forming a heterodimer and is highly expressed in liver and testis. The gene encoding PISD maps to human chromosome 22q12.2.

Subunit:
Heterodimer

Subcellular Location:
Mitochondrion.

Similarity:
Belongs to the phosphatidylserine decarboxylase family.

SWISS:
Q9UG56

Gene ID:
23761

Database links:

Entrez Gene: 23761 Human

Entrez Gene: 320951 Mouse

Omim: 612770 Human

SwissProt: Q9UG56 Human

SwissProt: Q8BSF4 Mouse

Unigene: 420559 Human

Unigene: 273765 Mouse



Product Picture
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PISD) Polyclonal Antibody, Unconjugated (SL8866R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PISD) Polyclonal Antibody, Unconjugated (SL8866R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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