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Product Name phospho-Calmodulin 1/2/3 (Ser102) Chinese Name 磷酸化钙调节素抗体 Alias p-CaM I (Ser 102); Calmodulin 1/2/3(phospho S102); Calmodulin 1/2/3; CALM 1; CALM 2; CALM 3; CALM; CALM1; CALM2; CALM3; CALML2; Calmodulin 1; Calmodulin1; CAM 1; CAM 2; CAM 3; CaM; CAM I; CAM1; CAM2; CAM3; CAMB; CAMC; CAMI; CAMIII; DD132; PHKD; Phosphorylase kinase delta; Phosphorylase kinase delta subunit; Calmodulin1; Calmodulin 1; Calmodulin-1; Calmodulin2; Calmodulin 2; Calmodulin-2; Calmodulin3; Calmodulin 3; Calmodulin-3; CALM1_HUMAN. Product Type Phosphorylated anti Research Area Tumour Cell biology immunology Signal transduction transcriptional regulatory factor Cell Surface Molecule Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Pig, Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 16kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthesised phosphopeptide derived from human CaM I around the phosphorylation site of Ser102: YI(p-S)AA Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Calmodulin consists of two glycoproteins, 34 and 39 kDa, sometimes designated epithelial antigen, epithelial specific antigen, and epithelial glycoprotein. The glycoproteins are located on the cell membrane surface and in the cytoplasm of virtually all epithelial cells with the exception of most squamous epithelia, hepatocytes, renal proximal tubular cells, gastric parietal cells and myoepithelial cells. Epithelial Calmodulin is found in the large majority of adenocarcinomas of most sites (50-100% in various studies; as well as neuroendocrine tumours, including small cell carcinoma. Renal cell carcinoma and hepatocellular carcinoma stain in about 30% of the cases. Calmodulin mediates the control of a large number of enzymes and other proteins by Ca(2+). Among the enzymes to be stimulated by the calmodulin Ca(2+) complex are a number of protein kinases and phosphatases. Calmodulin has four functional calcium binding sites.
Subunit:
Interacts with MYO1C and RRAD. Interacts with MYO10 (By similarity). Interacts with CEP97, CEP110, TTN/titin and SRY. Interacts with USP6; the interaction is calcium dependent. Interacts with CDK5RAP2. Interacts with SCN5A. Interacts with RYR1 and RYR2.
Subcellular Location:
Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Note=Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules.
Post-translational modifications:
Ubiquitination results in a strongly decreased activity.
Phosphorylation results in a decreased activity.
Similarity:
Belongs to the calmodulin family. Contains 4 EF-hand domains.
SWISS:
P0DP23
Gene ID:
801
Database links:
Entrez Gene: 801 Human
Entrez Gene: 12313 Mouse
SwissProt: P0DP23 Human
SwissProt: P0DP26 Mouse
钙离子调节素(calmodulin,CaM): 钙调蛋白(CaM),是由一条多肽链组成的单体蛋白。可结合4个Ca2+,胞浆Ca2+浓度≥10-2mmol/L时,Ca2+与CaM结合,构象改变而激活Ca2+ -CaM激酶。Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-CaM I (Ser 102)) Polyclonal Antibody, Unconjugated (SL8519R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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