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Product Name GPX1 Chinese Name 谷胱甘肽过氧化酶1 Alias Glutathione peroxidase 1; Cellular glutathione peroxidase; GPx 1; GPx-1; GPX1; GPX1_HUMAN; GSHPx-1; GSHPX1; MGC14399; MGC88245. literatures Specific References (11) | SL3882R has been referenced in 11 publications.Research Area Tumour Cardiovascular immunology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Sheep, (predicted: Rat, Dog, Pig, Cow, Horse, Guinea Pig, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 22kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse Glutathione Peroxidase 1: 51-150/201 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene product belongs to the family of glutathione peroxidase, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq].
Subunit:
Homotetramer. Interacts with MIEN1 (By similarity).
Subcellular Location:
Cytoplasm.
Similarity:
Belongs to the glutathione peroxidase family.
SWISS:
P11352
Gene ID:
2876
Database links:Entrez Gene: 461015 Chimpanzee
Entrez Gene: 2876 Human
Entrez Gene: 706732 Macaque Monkey
Entrez Gene: 14775 Mouse
Entrez Gene: 100438450 Orangutan
Omim: 138320 Human
SwissProt: Q0EFA0 Chimpanzee
SwissProt: P07203 Human
SwissProt: Q865R2 Macaque Monkey
SwissProt: P11352 Mouse
SwissProt: Q4AEI3 Orangutan
Unigene: 76686 Human
Unigene: 1090 Mouse
Unigene: 11323 Rat
保护红细胞中血红蛋白的氧化分解.Product Picture Sample: Spleen (Mouse) Lysate at 40 ug
Primary: Anti- GPX1 (SL3882R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-GPX1 antibody (SL3882R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at PBST. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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