Product Name	ATG4A
Chinese Name	自噬相关蛋白4A抗体
Alias	AI627006 ; APG4 autophagy 4 homolog A ; Apg4a ; ATG4 autophagy related 4 homolog A (S. cerevisiae) ; ATG4 autophagy related 4 homolog A ; ATG4A ; ATG4A_HUMAN ; Atg4al ; AUT like 2 cysteine endopeptidase ; AUT-like 2 cysteine endopeptidase ; Autl2 ; Autophagin 2 ; Autophagin-2 ; Autophagy related 4A cysteine peptidase ; Autophagy related cysteine endopeptidase 2 ; Autophagy related protein 4 homolog A ; Autophagy-related cysteine endopeptidase 2 ; Autophagy-related protein 4 homolog A ; AV169859 ; Cysteine protease ATG4A ; hAPG4A ; MGC107179 .
Research Area	Tumour  immunology  Apoptosis  transcriptional regulatory factor
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Cow, Rabbit, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	45kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ATG4A: 51-150/398
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	ATG4A is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Function: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Subcellular Location: Cytoplasm (Probable). Tissue Specificity: Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. Similarity: Belongs to the peptidase C54 family. SWISS: Q8WYN0 Gene ID: 115201 Database links: Entrez Gene: 115201 Human Entrez Gene: 666468 Mouse Omim: 300663 Human SwissProt: Q8WYN0 Human SwissProt: Q8C9S8 Mouse Unigene: 8763 Human Unigene: 102230 Mouse Unigene: 393320 Mouse
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG4A) Polyclonal Antibody, Unconjugated (SL3873R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG4A) Polyclonal Antibody, Unconjugated (SL3873R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ATG4A) Polyclonal Antibody, Unconjugated (SL3873R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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