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Product Name GMF beta Chinese Name 胶浆成熟因子β抗体 Alias Glia maturation factor beta; GMF. GMF-beta; GMFB; GMFB_HUMAN. Research Area Tumour Neurobiology Growth factors and hormones Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, Dog, Pig, Horse, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 17kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human GMF beta: 21-110/142 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Glia maturation factor β (GMF-β) belongs to the GMF subfamily of the larger actin-binding protein ADF family. This protein, which is phosphorylated following phorbol ester stimulation, is important for the nervous system. It causes brain cell differentiation, stimulates neural regeneration and inhibits tumor cell proliferation. Overexpression of GMF in astrocytes has been shown to enhance brain-derived neurotrohic factor (BDNF) production. GMF expression is increased by exercise, and the protein is crucial for exercise-induction of BDNF. Through BDNF production, GMF appears to play a role in neuroprotection. In thymoma, T-cell development is maintained by GMF-β being produced by the tumor cells.
Function:
This protein causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells.
Post-translational modifications:
Phosphorylated; stimulated by phorbol ester.
Similarity:
Belongs to the actin-binding proteins ADF family. GMF subfamily.
Contains 1 ADF-H domain.
SWISS:
P60983
Gene ID:
2764
Database links:Entrez Gene: 2764 Human
Omim: 601713 Human
SwissProt: P60983 Human
Unigene: 151413 Human
Product Picture Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- GMF beta (SL11845R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 19 kD
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GMF beta) Polyclonal Antibody, Unconjugated (SL11845R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GMF beta) Polyclonal Antibody, Unconjugated (SL11845R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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