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Product Name Chordin Chinese Name 原肠胚双向形成相关蛋白抗体 Alias Chordin; Chrd; CHRD_HUMAN; MGC133038; Shortgastrulation protein; SOG. literatures Specific References (1) | SL11831R has been referenced in 1 publications.[IF=7.815] Zhang Y et al. Directed Differentiation of Notochord-like and Nucleus Pulposus-like Cells Using Human Pluripotent Stem Cells. Cell Rep. 2020 Feb 25;30(8):2791-2806.e5. WB ; human.Research Area Cell biology Developmental biology Neurobiology Stem cells Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 99kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Chordin: 412-490/951 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The patterning of the CNS relies on the interaction of multiple signaling molecules such as Sonic Hedgehog, Wnts, and BMPs and their antagonists Chordin and Noggin. At midgastrula, expression of Noggin overlaps that of Chordin. Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta-like bone morphogenetic proteins and sequestering them in latent complexes. The gene which encodeschordin maps to human chromosome 3q27. Noggin is a secreted protein that binds and inactivates members of the transforming growth factor-beta (TGF-beta) superfamily of signaling proteins, such as BMP-2,4,7. The gene which encodes noggin maps to human chromosome 17q22.
Function:
Dorsalizing factor. Key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGF-beta family bone morphogenetic proteins (BMPs) and sequestering them in latent complexes.
Subunit:
Interacts with TWSG1 and/or BMP4
Subcellular Location:
Secreted.
Tissue Specificity:
Expressed at the highest level in liver.
Post-translational modifications:
Cleaved by tolloid proteases; cleavage participates to dorsoventral patterning during early development.
Similarity:
Belongs to the chordin family.
Contains 4 CHRD domains.
Contains 4 VWFC domains.
SWISS:
Q9H2X0
Gene ID:
8646
Database links:Entrez Gene: 8646 Human
Entrez Gene: 12667 Mouse
Omim: 603475 Human
SwissProt: Q9H2X0 Human
SwissProt: Q9Z0E2 Mouse
Unigene: 166186 Human
Unigene: 20457 Mouse
Unigene: 29459 Rat
Product Picture Sample:
HepG2(Human) Cell Lysate at 30 ug
U2OS(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Chordin (SL11831R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 96 kD
Sample:
HepG2(Human) Cell Lysate at 30 ug
U2OS(Human) Cell Lysate at 30 ug
MDA-MB-231(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- Chordin (SL11831R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 99 kD
Observed band size: 99 kD
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