Product Name	Engrailed 1
Chinese Name	同源盒蛋白转录因子EN1抗体
Alias	EN1 / Engrailed 1; Engrailed1; En 1; EN1; EN-1; Engrailed; Engrailed homeobox 1; Engrailed homolog 1; HME1_HUMAN; Homeobox protein en-1; Homeobox protein engrailed 1; Homeobox protein engrailed-1; Homeobox protein engrailed-1; Hu-En-1; Mo-en.1.
literatures	Specific References  (1)     |     SL11744R has been referenced in 1 publications. [IF=6.525] Mengfan Wu. et al. Continuous NPWT Regulates Fibrosis in Murine Diabetic Wound Healing. PHARMACEUTICS. 2022 Oct;14(10):2125  IF ;  Mouse.   PubMed:36297560
Research Area	Cell biology  Developmental biology  Neurobiology  Signal transduction  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	40kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human EN1/Engrailed 1: 351-420/392
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The engrailed-1 gene, EN1, a murine homolog of the Drosophila homeobox gene engrailed (EN), is required for midbrain and cerebellum development and dorsal/ventral patterning of the limbs as well as apical ectodermal ridge formation. In Drosophila, the EN gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Human EN-1 and EN-2 are homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Different mutations in the mouse homo-logs, EN-1 and EN-2, produce different developmental defects that frequently are lethal. EN-1 is highly expressed by essentially all dopaminergic neurons in the substantia nigra and ventral tegmentum. EN-1 and EN-2 regulate expres-sion of a-synuclein, a gene that is genetically linked to Parkinson’s disease. Subcellular Location: Nucleus. Similarity: Belongs to the engrailed homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: Q05925 Gene ID: 2019 Database links: Entrez Gene: 2019 Human Entrez Gene: 13798 Mouse Entrez Gene: 685360 Rat Omim: 131290 Human SwissProt: Q05925 Human SwissProt: P09065 Mouse Unigene: 271977 Human Unigene: 2657 Mouse Unigene: 162354 Rat
Product Picture	Sample: Lane 1: Muscle (Mouse) Lysate at 40 ug Lane 2: Cerebrum (Mouse) Lysate at 40 ug Lane 3: Muscle (Rat) Lysate at 40 ug Lane 4: Cerebrum (Rat) Lysate at 40 ug Primary: Anti-Engrailed 1 (SL11744R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43/52 kD Observed band size: 50 kD Blank control(blue): Raji Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Engrailed 1/AF647 Conjugated antibody (SL11744R-AF647), Dilution: 0.2μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.

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