Product Name	GCX1/C20orf100
Chinese Name	20号染色体开放阅读框100抗体
Alias	Chromosome 20 open reading frame 100; Chromosome 20 open reading frame 100; dJ1108D11.2; dJ495O3.1; GCX 1; GCX1; GCX-1; Granulosa cell HMG box protein 1; MGC15880; Weakly similar to NONHISTONE CHROMOSOMAL PROTEIN 6A [S.cerevisiae]; Weakly similar to NONHISTONE CHROMOSOMAL PROTEIN 6A [S.cerevisiae]; TOX2_HUMAN.
Research Area	Cell biology  Neurobiology  Signal transduction  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse,  (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	52kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human GCX1/C20orf100: 201-300/488
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	GCX-1 is a 488 amino acid transcription activator that is restricted to expression in the hypothalamus, pituitary, ovary, testis and uterus. Human reproduction is controlled by the hypothalamic-pituitary gonadal axis (HPGA) that is laid down early in fetal development. This expression pattern in the HPGA suggests that this nuclear protein is likely related to specific events in reproduction, particularly in the female. GCX-1 contains a HMG-box domain, which is commonly found in proteins that function as intercellular regulators and transcriptional co-regulators, and are found to be involved in important events such as sex determination and in the regulation of T cell differentiation. There are two isoforms of GCX-1 which are produced as a result of alternative splicing events. Function: C20orf100 is a putative transcriptional activator involved in the hypothalamo-pituitary-gonadal system. It may regulate follicular development and other processes related to female reproduction. Subcellular Location: Nuclear Similarity: Contains 1 HMG box DNA-binding domain. SWISS: Q96NM4 Gene ID: 84969 Database links: Entrez Gene: 84969 Human Entrez Gene: 269389 Mouse Entrez Gene: 311615 Rat Omim: 611163 Human SwissProt: Q96NM4 Human SwissProt: Q76IQ7 Rat Unigene: 26608 Human Unigene: 129021 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCX1 C20orf100) Polyclonal Antibody, Unconjugated (SL11402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GCX1 C20orf100) Polyclonal Antibody, Unconjugated (SL11402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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