Product Name	MRF/C11orf9
Chinese Name	髓鞘基因调控因子抗体
Alias	C11orf9; Chromosome 11 open reading frame 9; Gm1804; gm98; KIAA0954; MGC10781; MRF; MRF_HUMAN; Myelin gene regulatory factor; Ndt80.
literatures	Specific References  (1)     |     SL11191R has been referenced in 1 publications. [IF=3.47] Xiaowei Yu. et al. Nanophthalmos-Associated MYRF Gene Mutation Causes Ciliary Zonule Defects in Mice. Invest Ophth Vis Sci. 2021 Mar;62(3):1-1  WB,IHC ;  Mouse.   PubMed:33646289
Research Area	Cell biology  immunology  Neurobiology  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	124kDa
Cellular localization	The nucleus The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human MRF: 401-500/1151
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	C11orf9 (chromosome 11 open reading frame 9), also known as MRF (myelin gene regulatory factor), Ndt80, KIAA0954 or MGC10781, is a 1,151 amino acid single-pass membrane protein that localizes to nucleus. A member of the MRF family, C11orf9 is expressed in the ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. C11orf9 is weakly expressed in cerebellum and retina. C11orf9 contains one NDT80 DNA-binding domain and acts as a transcription factor that is required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. C11orf9 exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 11, which consists of approximately 135 million base pairs and 1,400 genes. Chromosome 11 makes up around 4% of human genomic DNA and is considered a gene and disease association dense chromosome. Function: Transcription regulator required for expression of central nervous system (CNS) myelin genes such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS myelination. Probably acts as a transcription factor that directly binds DNA and activates expression of CNS myelin genes. Subunit: Belongs to the MRF family. Contains 1 NDT80 DNA-binding domain. Subcellular Location: Membrane. Nucleus. Tissue Specificity: Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion and liver. Weakly expressed in cerebellum and retina. Similarity: Belongs to the MRF family. Contains 1 NDT80 DNA-binding domain. SWISS: Q9Y2G1 Gene ID: 745 Database links: Entrez Gene: 225908 Mouse SwissProt: Q3UR85 Mouse Unigene: 329716 Mouse
Product Picture	Sample: Brain (Mouse) Lysate at 40 ug Primary: Anti-MRF/C11orf9 (SL11191R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 124 kD Observed band size: 124 kD Sample: Uterus (Mouse) Lysate at 40 ug Primary: Anti- MRF/C11orf9 (SL11191R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kD Observed band size: 124 kD Sample: Lane 1: Human A549 cell lysates Lane 2: Human HepG2 cell lysates Lane 3: Human U2OS cell lysates Lane 3: Human A431 cell lysates Primary: Anti- MRF/C11orf9 (SL11191R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kDa Observed band size: 124 kDa Sample: Lung (Mouse) Lysate at 40 ug Primary: Anti- MRF/C11orf9 (SL11191R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kD Observed band size: 124 kD Sample: A549(Human) Cell Lysate at 30 ug Primary: Anti- MRF/C11orf9 (SL11191R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 124 kD Observed band size: 124 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-MRF/C11orf9 Polyclonal Antibody, Unconjugated(SL11191R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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