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Product Name LPLUNC1 Chinese Name 鼻咽癌癌基因抗体 Alias BPI fold containing family B, member 1; long palate, lung and nasal epithelium carcinoma associated 1 isoform 1; chromosome 20 open reading frame 114; C20orf114; Long palate; BPIB1_HUMAN; Lplunc1; lung and nasal epithelium carcinoma-associated protein 1; VEMSGP; Von Ebner minor salivary gland protein; MGC14597; MGC156708; RGD1563047; RP23-154J12.1; U46068. Research Area immunology Microbiology Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 50kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human LPLUNC1: 21-120/484 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene may be involved in the innate immune response to bacterial exposure in the mouth, nasal cavities, and lungs. The encoded protein is secreted and is a member of the BPI/LBP/PLUNC protein superfamily. This gene is found with other members of the superfamily in a cluster on chromosome 20. [provided by RefSeq]
Function:
May play a role in innate immunity in mouth, nose and lungs.
Subcellular Location:
Secreted (By similarity).
Tissue Specificity:
Detected in trachea, nasal septal epithelium and lung.
Similarity:
Belongs to the BPI/LBP/Plunc superfamily. Plunc family.
SWISS:
Q8TDL5
Gene ID:
92747
Database links:Entrez Gene: 92747 Human
SwissProt: Q8TDL5 Human
Unigene: 65551 Human
Product Picture Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti- LPLUNC1 (SL0976R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- LPLUNC1 (SL0976R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Sample:
Lane1: Lung(Rat) Lysate at 30 ug
Lane2:Colon carcinoma(Human) Lysate at 30 ug
Primary: Anti-LPLUNC1 (SL0976R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size: 50kD
Observed band size: 53kD
Tissue/cell: human Nasal polyps tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LPLUNC1 Polyclonal Antibody, Unconjugated(SL0976R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
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