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Product Name TTF1/NKX2-1 Chinese Name 甲状腺核转录因子-1抗体 Alias NKX21_HUMAN; Homeobox protein Nkx-2.1; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1 (TTF-1); Thyroid-specific enhancer-binding protein (T/EBP); NKX2A; TITF1; TTF1; TTF-1; NK2 homeobox 1; BCH; BHC; NK-2; TEBP; NMTC1; T/EBP; literatures Specific References (4) | SL0826R has been referenced in 4 publications.[IF=4.872] Dong X et al. PM2.5 disrupts thyroid hormone homeostasis through activation of the hypothalamic-pituitary-thyroid (HPT) axis and induction of hepatic transthyretin in female rats 2.5Ecotoxicol Environ Saf.2021 Jan 15;208:111720. IHC、WB ; Rat.[IF=4.223] Dong, Xinwen. et al. Protective effects of curcumin against thyroid hormone imbalance after gas explosion-induced traumatic brain injury via activation of the hypothalamic-pituitary-thyroid axis in male rats. ENVIRON SCI POLLUT R. 2022 May;:1-13 WB,IHC ; Rat.[IF=3.14] Huang, Huibin, et al. "Upregulation of thyroid transcription factor-1 and human leukocyte antigen class I in Hashimoto's disease providing a clinical evidence for possible triggering autoimmune reaction." European Journal of Endocrinology 164.5 (2011): 795-800. WB, IHC-P ; Human.[IF=1.38] Vadasz, Stephanie, et al. "Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers." Journal of Pediatric Surgery (2014). Human.Research Area Tumour Cell biology transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, Applications WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 38kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human TTF-1: 201-300/372 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TFF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Apr 2011]
Function:
Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
Subcellular Location:
Nucleus.
Tissue Specificity:
Thyroid and lung.
Post-translational modifications:
Phosphorylated on serine residues by STK3.
DISEASE:
Chorea, hereditary benign (BHC) [MIM:118700]: A rare autosomal dominant movement disorder, defined by early onset in childhood, a stable or non-progressive course of chorea, and no mental deterioration. Chorea is characterized by involuntary, forcible, rapid, jerky movements that may be subtle or become confluent, markedly altering normal patterns of movement. Note=The disease is caused by mutations affecting the gene represented in this entry.
Choreoathetosis and congenital hypothyroidism with or without pulmonary dysfunction (CAHTP) [MIM:610978]: An autosomal dominant disorder that manifests in infancy with neurological disturbances, hypothyroidism, and respiratory problems. It is characterized by movement abnormalities beginning with muscular hypotonia followed by the development of chorea, athetosis, dystonia, ataxia, and dysarthria. {ECO:0000269|PubMed:11854318, Note=The disease is caused by mutations affecting the gene represented in this entry.
Similarity:
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain.
SWISS:
P43699
Gene ID:
7080
Database links:Entrez Gene: 7080 Human
Entrez Gene: 21869 Mouse
Omim: 600635 Human
SwissProt: P43699 Human
SwissProt: P50220 Mouse
Unigene: 94367 Human
Unigene: 89972 Mouse
Unigene: 34265 Rat
同源结构域蛋白(Homeodomain Proteins)
TTF-1存在于肺和大脑的一些区域内;包括垂体,甲状旁腺和甲状腺旁细胞中也有表达。
TTF-1有调节甲状腺、肺和大脑的基因表达的功能。它在甲状腺中的分子靶点是甲状腺球蛋白、促甲状腺素受体和甲状腺过氧化酶。 TTF1在肺腺癌和肺神经内分泌Tumour(包括有肺小细胞癌)中是较好的标记物之一。其特异性和敏感性都很高,可以用于区分肺原发性、继发性腺癌、小部分胃肠道腺癌等。
Product Picture Sample:
Lane 1: Mouse Thyroid gland Lysates
Lane 2: Rat Thyroid gland Lysates
Primary: Anti-TTF1 (SL0826R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38kDa
Observed band size: 38kDa
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37∩ for 20 min;
Incubation: Anti-TTF1/NKX2.1 Polyclonal Antibody, Unconjugated(SL0826R) 1:200, overnight at 4∑C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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