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Product Name Megsin Chinese Name 丝氨酸蛋白酶抑制剂B7抗体 Alias SER-PINB7; MEGSIN; Mesangium predominant gene megsin; MGC120014; MGC120015; Serpin B7; Serpin peptidase inhibitor clade B (ovalbumin) member 7; TP55; SPB7_HUMAN. Research Area Tumour Cell biology Neurobiology Signal transduction Apoptosis Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, (predicted: Human, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 42kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Megsin: 301-380/380 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Serpin B7 is a serine proteinase inhibitor of the ovalbumin like B clade of serpins. It was first discovered in a search for agents responsible for thrombopoiesis, and maturation of megakaryocytes. SerpinB7 was identified as a protein that increased acetylcholine esterase stimulation from bone barrow progenitor cells, and injection of SerpinB7 in mice induced a 40% increase in platelets compared to controls. Shortly thereafter, in a search for kidney specific genes, SerpinB7 was described as a protein specific to mesangial cells, and was named megsin to indicate the tissue specificity of the protein.
Function:
Might function as an inhibitor of Lys-specific proteases. Might influence the maturation of megakaryocytes via its action as a serpin.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Predominantly expressed in mesangial cells.
Similarity:
Belongs to the serpin family. Ov-serpin subfamily.
SWISS:
O75635
Gene ID:
8710
Database links:Entrez Gene: 8710 Human
Omim: 603357 Human
SwissProt: O75635 Human
Unigene: 138202 Human
Unigene: 742388 Human
megsin是新近发现的蛋白质,属于丝氨酸蛋白酶抑制剂(Serpin)超家族,特异表达于肾小球系膜细胞,其表达异常在肾脏疾病进展中具有重要作用,megsin蛋白适量的表达,对维持人体正常的生理病理过程如凝血、纤维溶解、间质代谢、发育、血压、Tumour和炎症等是很重要的。在系膜细胞增生和系膜基质增多明显的IgA肾病肾小球中,megsin的表达显著上调。Product Picture Sample:
Lane 1: Ear (Rat) Lysate at 40 ug
Lane 2: Tongue (Rat) Lysate at 40 ug
Primary: Anti-Megsin (SL0815R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Megsin) Polyclonal Antibody, Unconjugated (SL0815R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Megsin ) Polyclonal Antibody, Unconjugated (SL0815R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (Rat tongue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Megsin) Polyclonal Antibody, Unconjugated (SL0815R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Megsin ) Polyclonal Antibody, Unconjugated (SL0815R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Megsin Polyclonal Antibody, Unconjugated(SL0815R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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