Product Name	Beta-lactoglobulin
Chinese Name	β-乳球蛋白抗体
literatures	Specific References  (4)     |     SL0814R has been referenced in 4 publications. [IF=7.514] Dan Bu. et al. Insight into the mechanism of d-allose in reducing the allergenicity and digestibility of ultrasound-pretreated α-lactalbumin by high-resolution mass spectrometry. Food Chem. 2021 Nov;:131616   PubMed:34815116 [IF=5.162] Yan-Hong Shaoet al. Glycation of β-lactoglobulin combined by sonication pretreatment reduce its allergenic potential. Int J Biol Macromol . 2020 Dec 1;164:1527-1535.  other ;   PubMed:32738325 [IF=4.946] Zhong J et al.Comparison of antigenicity and conformational changes to β-lactoglobulin following kestose glycation reaction with and without dynamic high-pressure microfluidization treatment.(2019)Food Chem. Apr 25;278:491-496.  Antigenicity analysis ;   PubMed:30583402 [IF=4.006] Xu-mei Wang. et al. Investigation of the mechanism underlying the influence of mild glycation on the digestibility and IgG/IgE-binding abilities of β-lactoglobulin and its digests through LC orbitrap MS/MS. Lwt Food Sci Technol. 2021 Mar;139:110506   PubMed:10.1016/j.lwt.2020.110506
Research Area	Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Sheep, Goat,  (predicted: Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	18kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	Beta-lactoglobulin
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	bov beta-lactoglobulin is the content of the milk in the category of a protein myosin. Protein contained in whole milk (3.7 percent) to 80% of the casein in the removal of the casein whey protein, 55% of the beta-lactoglobulin. Access to the crystallization of the isoelectric point = 5.2, the pH in this dual capacity under the main structure. The people in latex-free Beta-lactoglobulin. The beta-lactoglobulin and bov beta-lactoglobulinA-based. Function: Primary component of whey, it binds retinol and is probably involved in the transport of that molecule. Subcellular Location: Secreted. Tissue Specificity: Synthesized in mammary gland and secreted in milk. Post-translational modifications: Alternate disulfide bonds occur in equal amounts in all variants examined. Similarity: Belongs to the calycin superfamily. Lipocalin family. SWISS: P02754 Gene ID: 280838 Database links: Entrez Gene: 280838 Cow SwissProt: Q32P89 Cow Unigene: 385 Cow β-乳球蛋白是含于乳中的球蛋白类的一种蛋白质。牛乳中所含全蛋白（3.7％）的80％为酪蛋白，在除去酪蛋白的乳清蛋白质中55％为β-乳蛋白。 可获得其结晶，等电点=5.2，在此pH下主要成双量体结构。而人的乳液中不含β-乳球蛋白。 该β-乳球蛋白与牛β-乳球蛋白A型基因、牛β-乳球蛋白B型基因、山羊和绵羊β-乳球蛋白基因的同源性分别为98.55％，99.79％，90.70％和90.09％
Product Picture	Sample: Goat milk at 30ug; Primary: Anti- Beta-lactoglobulin (SL0814R) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 18kD Observed band size : 18kD Paraformaldehyde-fixed, paraffin embedded (sheep breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta-lactoglobulin) Polyclonal Antibody, Unconjugated (SL0814R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-AF488) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (sheep breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Beta-lactoglobulin) Polyclonal Antibody, Unconjugated (SL0814R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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