Product Name	IL12
Chinese Name	白介素12抗体
Alias	Interleukin-12 subunit alpha; IL-12; CLMF p35; CLMF1; CTL maturation factor (TcMF); Cytotoxic lymphocyte maturation factor 1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL 12 subunit p35; IL12A; Interleukin 12 alpha chain; Interleukin 12 p35; Interleukin 12A; Natural killer cell stimulatory factor 1; Cytotoxic lymphocyte maturation factor 35 kDa subunit; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1; IL-12A; IL12A_HUMAN; IL12A_MOUSE.
literatures	Specific References  (5)     |     SL0767R has been referenced in 5 publications. [IF=7.182] Tingting Guo. et al. Lepidium meyenii Walpers polysaccharide and its cationic derivative re-educate tumor-associated macrophages for synergistic tumor immunotherapy. Carbohyd Polym. 2020 Dec;250:116904  IF ;  Mouse.   PubMed:33049880 [IF=7.169] Yang, Lin. et al. MANF ameliorates DSS-induced mouse colitis via restricting Ly6ChiCX3CR1int macrophage transformation and suppressing CHOP-BATF2 signaling pathway. ACTA PHARMACOL SIN. 2023 Jan;:1-16  WB,IHC ;  Mouse.   PubMed:36635421 [IF=6.49] Taylor-Fishwick, D. A., et al. "Production and function of IL-12 in islets and beta cells." Diabetologia (2012): 1-10  Mouse.   PubMed:23052055 [IF=3.252] Guangcong Peng. et al. Intranasal administration of DHED protects against exhaustive exercise-induced brain injury in rats. Brain Res. 2021 Dec;1772:147665  WB ;  Rat.   PubMed:34562473 [IF=1.55] Yang, Lijuan, et al. "Effect of IL-17 in the development of colon cancer in mice." Oncology Letters 12.6 (2016): 4929-4936.  WB ;  Mouse.   PubMed:28101230
Research Area	Tumour  Cell biology  immunology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	22kDa
Cellular localization	The cell membrane Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse IL-12: 51-150/215
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	IL-12 protein is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Function: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Subunit: Heterodimer with IL12B; disulfide-linked. The heterodimer is known as interleukin IL-12. Subcellular Location: Secreted. Similarity: Belongs to the IL-6 superfamily. SWISS: P43431 Gene ID: 16159 Database links: Entrez Gene: 3592 Human Entrez Gene: 16159 Mouse Entrez Gene: 84405 Rat Omim: 161560 Human SwissProt: P29459 Human SwissProt: P43431 Mouse SwissProt: Q9R103 Rat Unigene: 673 Human Unigene: 103783 Mouse Unigene: 207199 Rat IL-12是新近发现的cell factor，具有多种生物学活性，尤其是在抗Tumour免疫和抗病毒免疫中有重要的作用。
Product Picture	Sample: Liver (Mouse) Lysate at 40 ug Spleen (Mouse) Lysate at 40 ug NIH/3T3 (Mouse) CellLysate at 30 ug RAW246.7 (Mouse) CellLysate at 30 ug Primary: Anti- IL12 (SL0767R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 35/36 kD Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug Lane 2: Blood cell (Mouse) Lysate at 40 ug Lane 3: Raw264.7 (Mouse) Cell Lysate at 30 ug Lane 4: Liver (Mouse) Lysate at 40 ug Lane 5: Spleen (Rat) Lysate at 40 ug Lane 6: Liver (Rat) Lysate at 40 ug Primary: Anti-IL12 (SL0767R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 35 kD Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (SL0767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL12) Polyclonal Antibody, Unconjugated (SL0767R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-IL-12 Polyclonal Antibody, Unconjugated(SL0767R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (blue line): Mouse spleen (blue). Primary Antibody (green line): Rabbit Anti- IL12 antibody (SL0767R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 70% ice-cold methanol overnight at 4℃. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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