Product Name	CDK6
Chinese Name	周期素依赖性激酶6抗体
Alias	CDK 6; CDK6; Cell division protein kinase 6; Crk 2; Crk2; Cyclin dependent kinase 6; Cyclin-dependent kinase 6; MGC59692; p40; PLSTIRE; Serine/threonine-protein kinase PLSTIRE; STQTL11; CDK6_HUMAN; PLSTIRE; Serine/threonine protein kinase PLSTIRE.
literatures	Specific References  (9)     |     SL0568R has been referenced in 9 publications. [IF=10.75] Tian Xiangdong. et al. Autophagy Inhibition Contributes to Apoptosis of PLK4 Downregulation-induced Dormant Cells in Colorectal Cancer. INT J BIOL SCI. 2023 May;19(9):2817-2834  WB ;  Human.   PubMed:37324947 [IF=5.168] He, Xiaoqin, et al. "Long non-coding RNA AK058003, as a precursor of miR-15a, interacts with HuR to inhibit the expression of Υ-synuclein in hepatocellular carcinoma cells." Oncotarget 8.6 (2017): 9451.  WB ;  Human.   PubMed:28035067 [IF=3.9] Zhang, Haifeng, et al. "The ClC-3 chloride channel protein is a downstream target of cyclin D1 in nasopharyngeal carcinoma cells." The international journal of biochemistry & cell biology 45.3 (2013): 672-683.   PubMed:23270726 [IF=3.74] Hu, Zheng, et al. "5-Aminolevulinic acid-mediated sonodynamic therapy induces anti-tumor effects in malignant melanoma via p53-miR-34a-Sirt1 axis." Journal of dermatological science 79.2 (2015): 155-162.  WB ;  Mouse.   PubMed:25982144 [IF=3.337] Yunping Lu. et al. Peroxiredoxin1 Knockdown Inhibits Oral Carcinogenesis via Inducing Cell Senescence Dependent on Mitophagy. Oncotargets Ther. 2021; 14: 239–251  IHC ;  Mouse.   PubMed:33469304 [IF=3.263] Fei Min et al. Hepatoprotective effects of hydroxysafflor yellow A in D-galactose-treated aging mice. Eur J Pharmacol. 2020 Aug 15;881:173214.  WB/IHC ;  Mouse.   PubMed:32454116 [IF=2.84] Yang, Ning, et al. "SOX 1, contrary to SOX 2, suppresses proliferation, migration, and invasion in human laryngeal squamous cell carcinoma by inhibiting the Wnt/β-catenin pathway." Tumor Biology (2015): 1-11.  WB ;  Human.   PubMed:26040764 [IF=2.233] Zhe Wang. et al. Baicalin coadministration with lithium chloride enhanced neurogenesis via GSK3β pathway in corticosterone induced PC-12 cells. 2022 Mar 15  WB ;  Rat.   PubMed:35296580 [IF=1.28] Zhang et al. miR-145 downregulates the expression of cyclin-dependent kinase 6 in human cervical carcinoma cells. (2014) Exp.Ther.Me. 8:591-594  WB ;  Human.   PubMed:25009625
Research Area	Tumour  Cell biology  Chromatin and nuclear signals  Signal transduction  Cyclin  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	37kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CDK6: 21-110/326
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This kinase is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression and G1/S transition. The activity of this kinase first appears in mid-G1 phase, which is controlled by the regulatory subunits including D-type cyclins and members of INK4 family of CDK inhibitors. This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. Function: Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and regulates negatively cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. Subunit: Interaction with D-type G1 cyclins. Cyclin binding promotes enzyme activation by phosphorylation at Thr-177. Binds to RUNX1, CDKN2D, FBXO7 and CDKN2C/p18-INK4c. Forms a cytoplasmic complex with Hsp90/HSP90AB1 and CDC37. FBXO7-binding promotes D-type cyclin binding. Interacts with Kaposi's sarcoma herpesvirus (KSHV) V-cyclin and herpesvirus saimiri (V-cyclin/ECLF2); the CDK6/V-cyclin complex phosphorylates NPM1 and thus lead to viral reactivation by reducing viral LANA levels. Subcellular Location: Cytoplasm. Nucleus. Cell projection, ruffle. Note=Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Tissue Specificity: Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells. Post-translational modifications: Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity. Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain. SWISS: Q00534 Gene ID: 1021 Database links: Entrez Gene: 1021 Human Entrez Gene: 12571 Mouse Entrez Gene: 114483 Rat Omim: 603368 Human SwissProt: Q00534 Human SwissProt: Q64261 Mouse Unigene: 119882 Human Unigene: 31672 Mouse Unigene: 389058 Mouse Unigene: 399714 Mouse Unigene: 162731 Rat Cyclin Dependent Kinase 6(CDK6)—周期素依赖性激酶6,是细胞周期在G1期向S期进展的关键因素之一.
Product Picture	Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-CDK6 (SL0568R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 35 kD Paraformaldehyde-fixed, paraffin embedded (human rectal carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDK6) Polyclonal Antibody, Unconjugated (SL0568R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDK6) Polyclonal Antibody, Unconjugated (SL0568R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDK6) Polyclonal Antibody, Unconjugated (SL0568R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDK6) Polyclonal Antibody, Unconjugated (SL0568R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDK6) Polyclonal Antibody, Unconjugated (SL0568R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDK6) Polyclonal Antibody, Unconjugated (SL0568R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-CDK6 antibody (SL0568R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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