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Product Name CCR-2 Chinese Name 细胞表面Chemokine受体2抗体 Alias C-C Chemokine receptor 2B; C C chemokine receptor type 2; C C CKR 2; CC chemokine receptor type 2; CC CKR 2; CCCKR2; CCR 2; CCR1L; CCR2A; CCR2B; CCR5L; CD192; CD192 antigen; Chemokine C C motif receptor 2; Chemokine CC Motif Receptor 2; CKR 2; CKR2; CKR2A; CKR2B; CMKBR2; MCP 1 R; MCP1 RECEPTOR; MCP1R; Monocyte chemoattractant protein 1 receptor; Monocyte Chemotactic Protein 1 Receptor; CCR2_HUMAN; C-C chemokine receptor type 2; C-C CKR-2; CC-CKR-2; CCR2; Monocyte chemoattractant protein 1 receptor; MCP-1-R. literatures Specific References (5) | SL0562R has been referenced in 5 publications.[IF=9.91] Li, Ting, et al. "Proliferation of parenchymal microglia is the main source of microgliosis after ischaemic stroke." Brain (2013): awt287. Mouse.[IF=5.17] Yang et al. CCL2-CCR2 axis promotes metastasis of nasopharyngeal carcinoma by activating ERK1/2-MMP2/9 pathway. (2016) Oncotarge. 7:15632-47 IHC ; Human.[IF=4.658] Liu T et al. High‐fat diet‐induced dysbiosis mediates MCP‐1/CCR2 axis‐dependent M2 macrophage polarization and promotes intestinal adenoma‐adenocarcinoma sequence. J Cell Mol Med. 2020 Feb;24(4):2648-2662. IHC-P&WB ; Mouse.[IF=4.137] Huang T et al. Schwann Cell-Derived CCL2 Promotes the Perineural Invasion of Cervical Cancer. Front Oncol. 2020 Jan 29;10:19. IHC-P ; Human.[IF=3.37] Lin-Peng Zhu. et al. Chemokine CCL7 mediates trigeminal neuropathic pain via CCR2/CCR3-ERK pathway in the trigeminal ganglion of mice. MOL PAIN. ;(): IF ; Mouse.Research Area immunology Cell Surface Molecule Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Dog, Horse, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=0.2μg/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 41kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CCR-2: 201-300/373 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes two isoforms of a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The receptors encoded by this gene mediate agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This gene is located in the chemokine receptor gene cluster region. Two alternatively spliced transcript variants are expressed by the gene. [provided by RefSeq, Mar 2009].
Function:
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
Subunit:
Binds to HIV-1 Tat. Interacts with ARRB1.
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Post-translational modifications:
N-glycosylated.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
P41597
Gene ID:
729230
Database links:Entrez Gene: 729230 Human
Entrez Gene: 12772 Mouse
Omim: 601267 Human
SwissProt: P41597 Human
SwissProt: P51683 Mouse
Unigene: 511794 Human
Unigene: 705362 Human
Unigene: 6272 Mouse
Unigene: 211983 Rat
CCR-2是属于G protein-coupled receptor一家族,参与调节白细胞的迁移和活性作用,是The cell membrane蛋白,它的变异与HIV-1抵抗有关。CCR-2不仅在宫颈癌组织中表达,而且在人其他Tumour如白血病、淋巴瘤、乳腺癌、肾B 癌、胃癌、结肠癌、肝癌和子宫癌中均有过度表达,可作为肝癌及乳腺癌诊断的Maker。Product Picture Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CCR2 Polyclonal Antibody, Unconjugated(SL0562R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control: Raw264.7.
Primary Antibody (green line): Rabbit Anti-CCR-2 antibody (SL0562R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control (blue line): Hela (blue).
Primary Antibody (green line): Rabbit Anti- CCR-2 antibody (SL0562R)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 80% methanol for 5 min at -20℃ (The cells were fixed with 2% paraformaldehyde for 10 min at room temperature). Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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