Product Name	CD10
Chinese Name	CD10抗体
Alias	NEP_HUMAN; Neprilysin; MME; membrane metalloendopeptidase; NEP; SFE; CD10; CALLA; CMT2T; SCA43; Atriopeptidase; Common acute lymphocytic leukemia antigen; CALLA; Enkephalinase; Neutral endopeptidase 24.11; Neutral endopeptidase; Skin fibroblast elastase;
literatures	Specific References  (3)     |     SL0527R has been referenced in 3 publications. [IF=3.439] Wang L et al. TGF-β1 stimulates epithelial–mesenchymal transition and cancer-associated myoepithelial cell during the progression from in situ to invasive breast cancer. Cancer Cell Int. 2019 Dec 19;19:343.  ICF ;  Human.   PubMed:31889895 [IF=2.349] Yasuno Yamaguchi. et al. Hyperthyroidism exacerbates ischemic reperfusion injury in the kidney. 2021 Oct 09  FC ;  Mouse.   PubMed:34629337 [IF=1.966] Tian L et al. Gingerol inhibits cisplatin-induced acute and delayed emesis in rats and minks by regulating the central and peripheral 5-HT, SP, and DA systems. J Nat Med. 2019 Nov 25.  IHC-P ;  rat&mink.   PubMed:31768887
Research Area	Tumour  Cell biology  immunology  Stem cells  Cell Surface Molecule  Cell type markers  lymphocyte  b-lymphocyte
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,  (predicted: Mouse, Rat, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3μg /test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	82kDa
Detection molecular weight	100kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CD10: 501-600/750 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a type II transmembrane glycoprotein and a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). The encoded protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. [provided by RefSeq, Aug 2017] Function: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Post-translational modifications: Myristoylation is a determinant of membrane targeting. Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity. Similarity: Belongs to the peptidase M13 family. SWISS: P08473 Gene ID: 4311 Database links: Entrez Gene: 4311 Human Entrez Gene: 17380 Mouse Entrez Gene: 24590 Rat Omim: 120520 Human SwissProt: P08473 Human SwissProt: Q61391 Mouse SwissProt: P07861 Rat Unigene: 307734 Human Unigene: 296022 Mouse Unigene: 33598 Rat CD10即共同型急性lymphocyte白血病抗原,是II型跨膜glycoprotein.CD10表达于早期Blymphocyte,某些粒性白细胞,骨髓基质细胞,部分上皮组织及其起源的Tumour细胞中也有表达.主要应用于某些恶性淋巴瘤和白血病的分型.CD10是一种94kD的锌依赖性The cell membrane金属Binding protein，参与细胞神经肽和激素肽的后分泌过程。它广泛地分布于肾、肝、小肠、胎盘、脉络膜丛、脑腺、腺皮质和白细胞，CD10是生发中心细胞及所致淋巴瘤的Maker。
Product Picture	Sample: Lane 1: Rat Kidney tissue lysates Lane 2: Human RAMOS cell lysates Primary: Anti-CD10 (SL0527R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 82 kDa Observed band size: 100 kDa Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-CD10 (SL0527R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 82 kD Observed band size: 82 kD Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD10) Polyclonal Antibody, Unconjugated (SL0527R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD10) Polyclonal Antibody, Unconjugated (SL0527R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (CD10) Polyclonal Antibody, Unconjugated (SL0527R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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