Product Name	CYP11A1
Chinese Name	细胞色素P450 11A1抗体
Alias	Cholesterol 20 22 desmolase； Cholesterol desmolase； Cholesterol monooxygenase (side chain cleaving)； Cholesterol side chain cleavage enzyme； Cholesterol side chain cleavage enzyme mitochondrial； Cholesterol side-chain cleavage enzyme； CP11A_HUMAN； CYP11A； CYPXIA1； Cytochrome P450 11A1； Cytochrome P450 11A1 mitochondrial； Cytochrome P450 family 11 subfamily A polypeptide 1； Cytochrome P450 subfamily XIA； Cytochrome P450(scc)； Cytochrome P450C11A1； mitochondrial； P450SCC； Steroid 20 22 lyase
literatures	Specific References  (16)     |     SL10099R has been referenced in 16 publications. [IF=10.588] Tianxin Zhao. et al. Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders. J Hazard Mater. 2022 Feb;424:127547  IHC ;  Mouse.   PubMed:10.1016/j.jhazmat.2021.127547 [IF=7.129] Hui Zhao. et al. Alleviating effects of selenium on fluoride-induced testosterone synthesis disorder and reproduction toxicity in rats. ECOTOX ENVIRON SAFE. 2022 Dec;247:114249  WB, IHC ;  Rat.   PubMed:36323150 [IF=6.208] Sijie Fan. et al. Seasonal Change in Adiponectin Associated with Ovarian Morphology and Function in Wild Ground Squirrels (Citellus dauricus Brandt). INT J MOL SCI. 2022 Jan;23(23):14698  IHC ;  Squirrel.   PubMed:36499026 [IF=5.201] Zhong Yuyi. et al. MIR143 Inhibits Steroidogenesis and Induces Apoptosis Repressed by H3K27me3 in Granulosa Cells. Front Cell Dev Biol. 2020 Oct;8:1159  WB ;  Porcine.   PubMed:33195195 [IF=4.522] Xiao L et al. Dihydrotestosterone synthesis in the sheep corpus luteum and its potential mechanism in luteal regression. J Cell Physiol. 2019 Jan 22.  WB ;  Sheep.   PubMed:30671954 [IF=4.2] Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).  IHC-P ;  Mouse.   PubMed:29202267 [IF=3.813] Wenqian Xie. et al. Seasonal expressions of SF-1, StAR and P450scc in the scent glands of the muskrats (Ondatra zibethicus). J Steroid Biochem. 2020 Nov;204:105766  IHC ;  Muskrat.   PubMed:32991988 [IF=3.738] Li, Wanpan. et al. Exposure to Fluoride From in Utero to Puberty Alters Gonadal Structure and Steroid Hormone Expression in Offspring Rats. BIOL TRACE ELEM RES. 2022 Apr;:1-13  WB ;  Rat.   PubMed:35445938 [IF=3.061] Wenju Liu. et al. High and low dose of luzindole or 4-phenyl-2-propionamidotetralin (4-P-PDOT) reverse bovine granulosa cell response to melatonin. PEERJ. 2023 Jan;11:e14612  WB ;  Bovine.   PubMed:36684672 [IF=2.984] Junjie Hu. et al. Assessment of progesterone synthesis and its regulation role on dihydrotestosterone secretion in sheep epididymis. Gene. 2021 Jul;790:145699  WB,IHC ;  Sheep.   PubMed:33964380 [IF=2.638] An SY et al. MiR-1197-3p regulates testosterone secretion in goat Leydig cells via targeting PPARGC1A. Gene. 2019 May 31;710:131-139.  WB ;  Goat.   PubMed:31158446 [IF=2.141] Huihui Wang. et al. Differential tissue expression of sex steroid-synthesizing enzyme CYP11A1 in male Tibetan sheep (Ovis aries). ANIM BIOTECHNOL. 2022 Sep 28  WB, IF ;  Sheep.   PubMed:36169054 [IF=2.094] Xiaodan Li. et al. Effects of SPATA6 on proliferation, apoptosis and steroidogenesis of Hu sheep Leydig cells in vitro. Theriogenology. 2021 May;166:9  WB ;  Sheep.   PubMed:33667862 [IF=2.083] Wenyang YU. et al. Seasonal change of circulating leptin associated with testicular activities of the wild ground squirrels (Citellus dauricus Brandt). INTEGR ZOOL. 2022 Jul;:  IHC ;  Squirrel.   PubMed:35841626 [IF=1.81] Ding Ziqiang. et al. Regulation of progesterone during follicular development by FSH and LH in sheep. ANIM REPROD. 2022 Jul;19:  IHC, WB ;  Sheep.   PubMed:35847559 [IF=1.537]  R. Fan . et al. Effects of the FABP4 gene on steroid hormone secretion in goose ovarian granulosa cells. Brit Poultry Sci. 2021;62(1):81-91  WB ;  Goose.   PubMed:32875818
Research Area	Tumour  Cell biology  immunology  transcriptional regulatory factor  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	53/57kDa
Cellular localization	cytoplasmic The cell membrane Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human CYP11A1/P450SCC: 41-140/521
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008] Function: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone. Subcellular Location: Mitochondrion membrane. DISEASE: Defects in CYP11A1 are the cause of adrenal insufficiency congenital with 46,XY sex reversal (AICSR) [MIM:613743]. A rare disorder that can present as acute adrenal insufficiency in infancy or childhood. ACTH and plasma renin activity are elevated and adrenal steroids are inappropriately low or absent; the 46,XY patients have female external genitalia, sometimes with clitoromegaly. The phenotypic spectrum ranges from prematurity, complete underandrogenization, and severe early-onset adrenal failure to term birth with clitoromegaly and later-onset adrenal failure. Patients with congenital adrenal insufficiency do not manifest the massive adrenal enlargement typical of congenital lipoid adrenal hyperplasia. Similarity: Belongs to the cytochrome P450 family. SWISS: P05108 Gene ID: 1583 Database links: Entrez Gene: 1583 Human Entrez Gene: 13070 Mouse Entrez Gene: 29680 Rat SwissProt: P05108 Human SwissProt: Q9QZ82 Mouse SwissProt: P14137 Rat Unigene: 303980 Human Unigene: 302865 Mouse Unigene: 1401 Rat
Product Picture	Sample:Testis(Mouse)Lysate at 40 ug Primary: Anti-CYP11A1(SL10099R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53/57 kD Observed band size: 53/57 kD Sample: Lane 1: Mouse Adrenal gland tissue lysates Lane 2: Rat Adrenal gland tissue lysates Primary: Anti-CYP11A1 (SL10099R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 53/57 kDa Observed band size: 47 kDa Paraformaldehyde-fixed, paraffin embedded (mouse adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome P450 family 11 subfamily A polypeptide 1) Polyclonal Antibody, Unconjugated (SL10099R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytochrome P450 family 11 subfamily A polypeptide 1) Polyclonal Antibody, Unconjugated (SL10099R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Cell: HepG2. Concentration:1:100 Host/Isotype:Rabbit/IgG Flow cytometric analysis of Rabbit IgG isotype control (Cat#: SL10099R) on HepG2(green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody .

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